FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768908
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT37150.48315273088587973No Hit
CTGTTAGCCTGTTGACAATAGTAGATTGCAAAATCCTCAGGCTGCAGGCTATTGATGGTGAGAGTGAAATCTGTC21360.27779656343801856No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18390.2391703558813278No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12820.16672995989117034No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12650.16451903218590522No Hit
GTATCAACGCAGAGTACGGGAAACTGTTAGCCTGTTGACAATAGTAGATT11540.1500829748162329No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10880.14149937313696828No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG9980.1297944617561529No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9670.12576277005831646No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9230.12004036893880674No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAGCAGCTGGTTAGTCTGGTATCAGCAGAAA8860.11522834981558261No Hit
GGATATTAGCAGCTGGTTAGTCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATGTATGCTGCATC8740.11366769496480723No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8670.1127573129685216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.11236714925582775No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT8490.11041633069235851No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC8270.10755513013260365No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7800.10144256530040005No Hit
CAATAGTAGATTGCAAAATCCTCAGGCTGCAGGCTATTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG7780.10118245615860415No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC7760.10092234701680824No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA7740.10066223787501234No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7730.10053218330411441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC251.8486132E-458.9892355
GTCGTTT200.007582631451.35785739
CTAATCG200.00890097849.3167719
TATGGCG1000.048.69930343
GTGGTAT4700.046.171891
CGCGTAT1150.044.65900439
TCGTATG9500.044.48361240
CGTATGC10450.044.22755441
TATACAC16000.043.357663
TGCTTGA9550.043.23818255
AAAGGGG9200.043.05922368
CTTGAAA10750.043.01341657
TTATACA16000.042.9466862
GCCGTCT9350.042.66827446
TGTGCGT601.8704122E-742.57819453
ACGTCAT14150.042.5316232
TCGACGT16150.042.51117329
CGACGTC16300.042.42923730
ACTCGAC16300.042.3961727
CTTATAC16200.042.2162781
Produced by FastQC (version 0.11.5)