FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530038
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA86250.563711489518561No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT68190.44567520545241357No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA66050.43168862472696756No Hit
GCTTAGGAGGCTGCCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT59610.38959816684291504No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54740.3577688920144467No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49160.32129920956211544No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43830.2864634734562148No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA39710.2595360376670383No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38960.2546341986277465No Hit
CTCCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATACTCCGCTCACTTT37850.24737947684959458No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36500.2385561665788693No Hit
ATGATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA36130.23613792598615196No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33430.21849130544470138No Hit
GAGTATCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGAAAT32000.20914513234311827No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31260.20430865115768368No Hit
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATCTGCAGATACA30120.1968578558179601No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30080.1965964244025312No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29470.19260959531724048No Hit
GTTTATTACTGTCAGCAATATTATGATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA28860.1886227662319498No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28570.1867273884700903No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28380.185485589246803No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28240.18457057929280188No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27610.18045303449979674No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC27600.18038767664593952No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG27290.17836158317636555No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27190.1777080046377933No Hit
ATATTATGATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT27120.17725049966079273No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC26900.1758126268759338No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGAAATCTGTCCCAGACCCGCTGCCA26730.17470154336036098No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCTC26400.1725447341830726No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25750.16829647368235298No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25460.16640109592049346No Hit
GATTTCACTCTCTCCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATACT25350.165682159528064No Hit
GTAAATGAGCAGCTTAGGAGGCTGCCCTGGTTTCTGCTGGTACCAAGCTA25020.1635253503507756No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24620.1609110361964866No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24290.15875422701919822No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT23860.15594383930333755No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23770.15535561861862254No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23540.15385238797990639No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA23090.1509112845563313No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGCCCTGGTTTCTGCTGGTA22680.1482316125481851No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22210.14515979341689553No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC22200.1450944355630383No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCTCCATCAGCAGCC21550.1408461750623187No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC20630.13483325250745407No Hit
GGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAA19880.12993141346816223No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19740.12901640351416108No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19650.12842818282944607No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTA19490.12738245716773047No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19370.12659816292144377No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19260.12587922652901432No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19150.12516029013658483No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19010.12424528018258371No Hit
GCAGAAACCAGGGCAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT18910.12359170164401145No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18870.12333027022858256No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18610.12163096602829473No Hit
TTATGATACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT18450.12058524036657912No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18290.11953951470486353No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACGCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG18180.11882057831243406No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18140.11855914689700517No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18080.11816699977386183No Hit
TCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGAAATCTGTC17800.11633697986585954No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17580.11489910708100061No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17350.11339587644228445No Hit
GTATCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGAAAT17290.1130037293191411No Hit
CTACGGGGACATCGTGATGACGCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG17020.11123906726499602No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGGCAGCCTCCTAAGCTGCTCATT16940.11071620443413824No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGGCAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATC16840.11006262589556601No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGAGAGAGTGAAATCTGTCCCAGACCCGCTGCCA16580.10836332169527815No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16450.10751366959513423No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16430.1073829538874198No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16350.106860091056562No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16260.10627187037184699No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATCTGCAGATA16220.10601043895641807No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16180.10574900754098918No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16030.10476863973313082No Hit
CTATATTACTGTGCGAAAGGGGGAACTGGAACTGATGATGCCTTTGATAT15900.10391898763298689No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATACTCCGC15690.10254647270198519No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15650.10228504128655627No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT15640.10221968343269905No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15580.1018275363095557No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15570.10176217845569849No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15530.10150074704026958No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15320.10012823210926787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGT258.13585E-6110.7534570
CGGATCG200.006466039553.47165360
GCCTAAT200.00716734252.09738546
ATACGCT200.007254651351.93810344
ACTCGAT304.4341173E-449.41547867
GTGGTAT2750.047.0639841
GGTATCA11900.046.080451
TGGTATC2850.044.2146572
GACAGGC2250.039.35761
GCGGTAT350.001435850638.92511
TCGATTG450.001442555838.8875169
CGTATGC2700.037.05233841
ATGCCGT2800.035.86202244
TCGTATG2700.035.71240
GAGACCG3000.035.20743624
GTATGCC3050.035.10669742
GTATCAA26550.034.893311
CTCGATT450.00248379534.81355368
CGAGGCT3050.034.67902829
AGACCGA3150.034.6204625
Produced by FastQC (version 0.11.5)