FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741275
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC93570.5373648619546022No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT59290.3404976238675683No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC53000.3043746679875379No Hit
GTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC49800.2859973295430073No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC46380.26635654908041523No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45130.2591779012505205No Hit
GACCTAAGACGGTGACCCTGGTCCCAACTCCGAAGACGTATCGGCCGTTGCCTAGATACGAAGTACAATAATAAT40850.2345982110809608No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG39820.2286830052691275No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC38060.21857546912463569No Hit
TCTCTGGGCTCCAGGCTGATGATGAGGCTGATTATTATTGTACTTCGTAT35120.2016912894287232No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG35030.2011744267849708No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33550.1926749077543754No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT33150.19037774044880906No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACGGTGACCCTGGTCCCAA32750.18808057314324272No Hit
TCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG30990.17797303699875092No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30860.17722645762444186No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28660.16459203744382708No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG27780.1595382693715812No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27100.15563308495211842No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACAGGAACTGGCGGTGATGTT27050.15534593903892263No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC26130.15006245423612008No Hit
GCCTTGGGCTGACCTAAGACGGTGACCCTGGTCCCAACTCCGAAGACGTATCGGCCGTTGCCTAGATACGAAGTA25440.14609984063401818No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23990.13777260915134024No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACAGGAACTGG23900.13725574650758784No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC23580.13541801266313477No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23340.134039712279795No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC23160.13300598699229013No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC22750.13065139050408464No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC22320.12818193565060085No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA21900.1257699099797562No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21630.12421932204849893No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA20630.11847640378458313No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG20620.11841897460194398No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA20490.11767239522763494No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG20130.11560494465262523No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT20120.11554751546998608No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19990.11480093609567701No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG19740.11336520652969807No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG19730.11330777734705891No Hit
TTATTGTACTTCGTATCTAGGCAACGGCCGATACGTCTTCGGAGTTGGGACCAGGGTCACCGTCTTAGGTCAGCC19650.11284834388594564No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT19570.11238891042483239No Hit
ACCTAAGACGGTGACCCTGGTCCCAACTCCGAAGACGTATCGGCCGTTGC19220.11037888903246183No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18920.1086560135532871No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT18870.10836886764009131No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACC18830.10813915090953467No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC18800.1079668633616172No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC18310.10515283341229846No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC18000.10337252875048455No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG17800.10222394509770141No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG17700.10164965327130983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21900.043.3910681
GTATCAA61650.041.486241
TGGTATC6700.040.2240372
GTGGTAT7000.038.5112231
TATCAAC75400.033.374882
ATCAACG77050.032.6498453
TCAACGC77550.032.292354
AACGCAG78400.032.0655146
CAACGCA78300.031.935465
CGCAGAG81950.030.6764648
ATCGTAC553.768865E-430.59345410
GTAGTTT4250.030.36437470
ACGCAGA82850.030.3026087
AGAGTAC84500.030.10571111
CAGAGTA84550.029.9312910
GCAGAGT84650.029.7377629
CGAACTA3050.027.73001129
TACGTAC2200.027.53489912
ATACGTA2250.026.9199211
GAGTACG65150.026.60274512
Produced by FastQC (version 0.11.5)