FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819632
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC48970.2691203496091517No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47120.25895345872132386No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG46360.2547767900322703No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC44890.2466982334889692No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC38820.21333984014350155No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTGAGTGTCACTGCTTGTATATGAGCTGCAGTAATAAT38150.20965777695709903No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA36910.20284321225390628No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35220.19355562003745813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35160.19322588303569074No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34610.19020329385282297No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32700.17970666596322774No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30570.16800100240048538No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29680.16310990354093574No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG29250.16074678836160278No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA28650.15744941834392887No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG28490.15657011967254916No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT28430.15624038267078177No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27850.15305292498703033No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTGAGTGTCACTGC27410.15063485364073614No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC27120.14904112479886045No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGCTTGATTATTACTGCAGCTCATAT27010.1484366069622869No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC26330.14469958760892312No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26010.1429409902661637No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG25080.13783006673876916No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24940.13706068040131192No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG24890.13678589956650575No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTAGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC23460.12892716769104962No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22460.12343155099492643No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21910.1204089618120587No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTGAGTGTCACTGCTTGTATATGAGCTG21870.12018913714421378No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTTGAGTGTCA21450.11788097813184203No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC21420.11771610963095835No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC20950.11513316978378045No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG20790.11425387111240076No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC20590.11315474777317612No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20020.1100222462563859No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC19460.10694470090655693No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19460.10694470090655693No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTAGTTATAACTATGTCTCCTGGTACCAACAACA19160.10529601589771997No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT18770.10315272538623194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT350.001449235738.85206615
GTATCAA51750.038.66831
GTGGTAT6600.034.5476881
TATCAAC59100.033.6132352
GGTATCA28350.032.651561
ATCAACG62350.031.6843973
TCAACGC62600.031.5991444
CAACGCA63650.031.1829535
AACGCAG63950.030.9835246
ACGCAGA67350.029.5203237
CGCAGAG67850.029.352878
AGAGTAC69150.028.8462311
CAGAGTA70450.028.60491810
GCAGAGT70900.028.186039
AGGATGG10900.027.96739669
GTGAGCG5200.027.3875570
CGTACCA1750.027.19495414
AGAGTCG650.00832454427.18792769
ATACGTA1750.027.18523811
TACGTAC1850.025.71929712
Produced by FastQC (version 0.11.5)