FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323462
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT39841.2316748180620907No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACT29340.9070617259523529No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCC27830.8603792717537145No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATG26230.8109144196227068No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAA22240.6875614446210065No Hit
ATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAA20110.6217113602216026No Hit
CGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGG17160.5305105391050571No Hit
GAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTC14920.4612597461216465No Hit
GTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGG14840.45878650351509603No Hit
GGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACATAGACAAGGAAC13460.41612306855210196No Hit
TTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCC13400.41426813659718914No Hit
GAACCGCACTCCTCGCCACACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGC12760.3944821957447861No Hit
GTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAG12600.38953571053168534No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAA11410.3527462267592484No Hit
CTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAA11310.3496546735010604No Hit
GGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTG11040.34130747970395287No Hit
GTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGC10780.3332694412326641No Hit
GGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCA10580.3270863347162882No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA9830.3038996852798783No Hit
GAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTT9170.28349543377583764No Hit
GTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTT9120.28194965714674364No Hit
ACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAG9000.27823979323691805No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTT8890.2748390846529113No Hit
CCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCA8720.2695834441139917No Hit
CTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTC8600.2658735802041662No Hit
CTATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCA8540.26401864824925336No Hit
ATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGA8400.2596904736877902No Hit
CCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTG8350.2581446970586962No Hit
CACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACA7950.2457784840259443No Hit
AGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCG7560.2337214263190112No Hit
CAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTCACCCTCTATGACGTC7520.232484805015736No Hit
CTCTATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTGCCCTCTCCAAATTACAACTCGGG7490.2315573390382796No Hit
GAACATAGACAAGGAACGGCCCCAAAGTTGCCCTCTCCAAATTACAACTC7420.22939325175754802No Hit
CTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTCACCCTCTATGA7400.22877494110591043No Hit
ACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTA7370.22784747512845402No Hit
AATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC7270.22475592187026608No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG6960.21517210676988333No Hit
GAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCT6920.21393548546660815No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC6810.21053477688260136No Hit
GCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTC6780.20960731090514498No Hit
GCCCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAG6720.20775237895023216No Hit
GCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGT6710.2074432236244134No Hit
CACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGG6130.18951221472692312No Hit
CCTCGCCACACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTG6120.18920305940110432No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCG5910.18271079755890957No Hit
GGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTC5890.18209248690727195No Hit
GTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTC5860.18116502092981557No Hit
ATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTGCC5820.17992839962654036No Hit
ACAAAGAACCGCACTCCTCGCCACACGGGATTCTCACCCTCTATGACGTC5710.1765276910425336No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC5450.16848965257124485No Hit
ACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTCACCCTCTATGACGTC5360.16570725463887567No Hit
GTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTGCCCTCTCCAAAT5210.1610699247515937No Hit
GTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAA5180.1601424587741373No Hit
GTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTGCCCTCTC5180.1601424587741373No Hit
CCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTC5050.15612343953849292No Hit
CCCTCTATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAA4900.15148610965121095No Hit
GTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAAT4800.14839455639302299No Hit
ATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCT4720.1459213137864726No Hit
TAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCT4650.14375722650574102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.13788327531518385No Hit
CTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCA4440.13726496466354626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.13695580933772744No Hit
TATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAA4400.13602834336027106No Hit
ACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGA4310.13324594542790189No Hit
CTCCTCGCCACACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAA4270.13200932412462668No Hit
GTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGT4250.1313910134729891No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGG4210.1301543921697139No Hit
GCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTCACC4140.1279903048889823No Hit
CTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGAGCT4050.12520790695661313No Hit
GTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGG3970.12273466435006276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.116242402507868No Hit
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGAC3480.10758605338494166No Hit
GACAAGGAACGGCCCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGAG3380.1044945001267537No Hit
GAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCT3380.1044945001267537No Hit
ATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTGCCCTCTCCAAATTA3370.10418534480093489No Hit
TCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTCAC3310.10233041284602211No Hit
GAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTC3300.1020212575202033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)