FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323462
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT35601.1005929599149205No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCC26120.8075137110387001No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACT24070.7441368692458465No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATG22060.6819966487562681No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18880.5836852551458904No Hit
GTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGG16570.5122703748817481No Hit
ATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAA16360.5057781130395533No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAA16280.5033048704330029No Hit
GTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAG14840.45878650351509603No Hit
CGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGG14220.43961887331433064No Hit
GGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCA13880.4291075922364915No Hit
GAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTC13430.41519560257464555No Hit
GGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACATAGACAAGGAAC13230.4090124960582696No Hit
GTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGC12990.40159276823861845No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAA12600.38953571053168534No Hit
GGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTG11810.3651124397920003No Hit
ACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAG10210.31564758766099266No Hit
AATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC9600.29678911278604597No Hit
GAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTT9600.29678911278604597No Hit
GGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTC8700.2689651334623541No Hit
CCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTG8360.258453852384515No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGG8360.258453852384515No Hit
GTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTT8110.2507249692390451No Hit
TTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCC7930.2451601733743067No Hit
CTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAA7790.24083199881284353No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTT7720.23866791153211195No Hit
ACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTA7460.23062987306082325No Hit
CTATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCA7450.23032071773500443No Hit
GCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGT7430.22970240708336684No Hit
ATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGA6990.21609957274733974No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC6660.2058974469953194No Hit
CCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCA6600.2040425150404066No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCAC6420.19847771917566823No Hit
AGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCG6320.19538616591748026No Hit
CTCTATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTGCCCTCTCCAAATTACAACTCGGG6190.1913671466818359No Hit
GAACATAGACAAGGAACGGCCCCAAAGTTGCCCTCTCCAAATTACAACTC6070.18765728277201033No Hit
CACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACA5950.18394741886218474No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCG5860.18116502092981557No Hit
GTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTC5810.17961924430072157No Hit
GAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCT5550.17158120582943281No Hit
GCCCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAG5320.16447063333560047No Hit
GCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTC5320.16447063333560047No Hit
CTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTC5240.1619973907290501No Hit
GTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTGCCCTCTC5160.1595241481224997No Hit
CACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGG5070.15674175019013054No Hit
GTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAAT4990.15426850758358013No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA4890.15117695432539216No Hit
ATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTGCC4890.15117695432539216No Hit
GAACCGCACTCCTCGCCACACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGC4850.14994033302211696No Hit
GTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAA4780.14777624574138537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.146848779763929No Hit
TAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCT4720.1459213137864726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.145303003134835No Hit
CAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTCACCCTCTATGACGTC4650.14375722650574102No Hit
ATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGG4460.13788327531518385No Hit
GTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGT4360.13479172205699586No Hit
GTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTGCCCTCTCCAAAT4310.13324594542790189No Hit
CCCTCTATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAA4290.13262763477626427No Hit
CCTCGCCACACGGGATTCTCACCCTCTATGACGTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTG4190.1295360815180763No Hit
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGAC3800.11747902381114321No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.11315084924968004No Hit
GAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTC3620.11191422794640483No Hit
CTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGAGCT3540.10944098533985445No Hit
CCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTC3520.10882267468821687No Hit
TATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATTCCCAAA3480.10758605338494166No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.10387618947511608No Hit
ACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTCACCCTCTATGACGTC3340.10325787882347849No Hit
CTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCA3320.1026395681718409No Hit
ATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCT3290.10171210219438451No Hit
ACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGA3290.10171210219438451No Hit
TTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCC3250.10047548089110932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACA150.001873461373.0870154
AGGATAG150.001909371572.7381553
TTCCTAC150.00204318471.5069548
GCTTCGC201.055899E-466.049841
TGTCCTG150.002814759765.9579544
GTTTTTC200.004756350557.7742464
CTTGGGC301.1868266E-554.9904632
ACTAGGG250.00619273954.04458669
GTGGTAT2300.053.127051
ACAGCGC253.1820443E-452.8398781
GTCCCGG253.1820443E-452.8398781
CTTCTAT501.6516424E-952.7908442
TGTGCGG253.203998E-452.766364
GCCCTGT200.00707215352.26384442
GGGCCCT200.007359632851.73993340
AGAATGT200.00876757649.49907318
AGCTATG200.00878366749.4761123
CGGGCCT401.125476E-649.4684684
GGGATCA200.00878903649.4684685
TGAGTAC850.046.5585564
Produced by FastQC (version 0.11.5)