FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912853
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATCTGCAAATACA29010.317794869491583No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT23460.25699647150198335No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21610.23673033883878347No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC20310.22248927264302137No Hit
ACTACATACCACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATTTG18970.207810019795082No Hit
GATTATTACTGCAGCTCATATGCAAGCAGCAGCACTCTCGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT18350.20101812668633393No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG18120.1984985534363145No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17700.1938975935884529No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16650.1823951939687989No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15930.1745078342296076No Hit
GTACCAACACCACCCAGGCACAGCCCCCAAACTCATGATTTATGAGGTCA15350.16815412777303684No Hit
GTATATTACTGTGCGAAAGGCGCCCTTTGGGGTTCGGGGACTTATTATGTTTTAAATTTCTTCGACCCCTGGGGC15320.16782548778390388No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14760.16169087465342175No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14610.16004767470775688No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACGAGAGTGCTGCTGCTTGCATATGAGCTGCAGTAATAAT14560.15949994139253526No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13960.15292714160987586No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT13900.1522698616316099No Hit
GTATGTAGTAGCACCACTAGCACTTGCAGCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCC13730.15040756835985641No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG13650.14953119505550183No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC13570.14865482175114722No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT13440.14723071513157102No Hit
CCCAGGCACAGCCCCCAAACTCATGATTTATGAGGTCACTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGG12650.13857652875106943No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACACCA12360.13539967552278406No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACGAGAGTGCTG12320.13496148887060677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12310.13485194220756244No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12260.13430420889234082No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12240.1340851155662522No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG12170.1333182889249419No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT11660.1277314091096814No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11600.12707412913141547No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACGAGAGTGCTGCTGC11160.12225407595746521No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCCATGCCATGGGCTGGGTCCGCCAGGCTCCAGGGAAGGGG10980.12028223602266738No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10870.11907722272917984No Hit
GTGTTGGTACCAGGAGACATAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA10810.11841994275091389No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACACCACCCAGGCACAGC10650.11666719614220472No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10450.11447626288131824No Hit
GGGTTCCCTGGCCCCAGGGGTCGAAGAAATTTAAAACATAATAAGTCCCC10430.11425716955522959No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10400.11392852956609663No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG10320.11305215626174203No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT10230.11206623629434313No Hit
GGTTATAACTATGTCTCCTGGTACCAACACCACCCAGGCACAGCCCCCAAACTCATGATTTATGAGGTCACTAAT10100.11064212967476691No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACGAGAGTGCTGCTGCTTGCATATGAGCTG10040.10998484969650098No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG9980.10932756971823504No Hit
GTATTTGCAGATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACT9490.10395978322906317No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATCTGCAAATA9390.10286431659861993No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9230.10111156998991076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2900.073.2510770
AATTCGA200.006960451652.48297552
ATTACGT200.007196383652.0424345
CGCGTAT200.00737035351.7289939
GGTATCA10350.046.2566641
TCTCGTA2600.045.03734238
CGTATGC3850.043.9909241
TGGTATC2600.043.401372
TCGACGT5000.043.15666229
CTTATAC5150.042.497111
ACTCGAC5100.042.28260427
CTCGACG5150.041.87668628
ATCTCGT2900.041.51550337
GACTCGA5200.041.45583726
CGACGTC5150.041.25270530
AGACTCG5250.041.0430225
TCGTATG3800.040.8861240
TTATACA5450.040.783052
TATACAC5300.040.646913
GCGTATG602.892466E-740.280440
Produced by FastQC (version 0.11.5)