FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912853
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51840.567889901221774No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATCTGCAAATACA27910.30574473655670736No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20360.223037005958243No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC18970.207810019795082No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC17950.19663626016456098No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT16560.18140927400140003No Hit
ACTACATACCACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATTTG16390.1795469807296465No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG15400.16870186108825846No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT14930.16355316792517524No Hit
GATTATTACTGCAGCTCATATGCAAGCAGCAGCACTCTCGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT14370.1574185547946931No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACGAGAGTGCTGCTGCTTGCATATGAGCTGCAGTAATAAT14330.15698036814251584No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13620.14920255506636884No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG13610.14909300840332454No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12800.14021972869673432No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACGAGAGTGCTG12690.13901471540324672No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC12680.13890516874020242No Hit
GTATATTACTGTGCGAAAGGCGCCCTTTGGGGTTCGGGGACTTATTATGTTTTAAATTTCTTCGACCCCTGGGGC12340.1351805821966954No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC12270.13441375555538515No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT12100.13255146228363165No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT11800.12926506239230193No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT11690.12806004909881438No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACGAGAGTGCTGCTGCTTGCATATGAGCTG11640.12751231578359276No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG11380.12466410254444035No Hit
GTATGTAGTAGCACCACTAGCACTTGCAGCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCC11310.12389727590313007No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCCATGCCATGGGCTGGGTCCGCCAGGCTCCAGGGAAGGGG11200.12269226260964253No Hit
GTACCAACACCACCCAGGCACAGCCCCCAAACTCATGATTTATGAGGTCA11110.1217063426422436No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACACCACCCAGGCACAGC11060.12115860932702198No Hit
GGGTTCCCTGGCCCCAGGGGTCGAAGAAATTTAAAACATAATAAGTCCCC10960.12006314269657875No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10590.11600991616393878No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10530.11535263618567283No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10340.11327124958783069No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT10010.109656209707368No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACGAGAGTGCTGCTGC9920.10867028973996909No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACACCA9820.10757482310952585No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG9720.10647935647908262No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9290.1017688499681767No Hit
GTGTTGGTACCAGGAGACATAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGA9170.10045429001164481No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG9150.10023519668555617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGA502.308191E-456.39338770
ACGCTAG200.007441043451.6042537
TGGTATC3650.047.726982
TCTGGAG11200.043.42794470
GGTATCA11950.040.0303761
GTATCAA31000.038.687891
CTACCGA400.002081332536.08938666
GCTAAGT400.002368788735.15038356
TAAAGCG400.00250082334.7638647
TATCAAC36000.033.3984872
ATCAACG37100.032.760343
TCAACGC37350.032.5267944
CAACGCA38100.031.8847565
AACGCAG38450.031.7720156
GAGTTAA450.004845513530.3722551
CGCAGAG40800.030.1092858
TATACGG11350.029.7644215
ATACGGC11250.029.7256726
GACAGGT1850.029.5513841
TACGGCC11250.029.4223487
Produced by FastQC (version 0.11.5)