FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474444
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC28100.1905803136639981No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27970.18969862538014332No Hit
CCACAGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCC27200.18447631785269566No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC26320.1785079663927555No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24500.16616433041878836No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23350.1583647802154575No Hit
GTATAATAACTGGCCTCCGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT21590.14642807729557716No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT21410.1452072781333167No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAACAGCTTCCAAGCACAGC21170.14357954591696936No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20020.1357799957136385No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20000.13564435136227623No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19660.1333383973891175No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG19580.13279581998366843No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16980.1151620543065725No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16870.11441601037408No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGAACCACTC16860.11434818819839884No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16630.11278827815773267No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16540.11217787857660244No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16490.11183876769819674No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15490.10505655013008293No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGACGGTGAGAGTGAACT15410.10451397272463384No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15240.10336099573805449No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14810.10044464218376555No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14790.10030899783240327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG5850.043.56674640
CGTATGC5850.043.04908841
GGTATCA13850.041.934591
ATGCCGT6100.040.939344
ATCTCGT5150.039.2867137
ACGAGAC6700.039.02596322
GACTAGG6900.038.90808526
GTATGCC6850.037.8474442
CTTATAC7000.037.3610231
CGAGACT7200.037.2616223
CCGTCTT6850.037.1653947
GTATCAA32400.036.6901171
TATACAC7550.036.4363373
TAGGCAT7350.036.12859729
CACGAGA7250.036.06292321
ATCTCCG7350.036.03058210
CGTCTTC7200.035.90564348
TATGCCG7250.035.3496443
TTATACA7400.035.339112
CCCACGA7450.035.09241519
Produced by FastQC (version 0.11.5)