FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474444
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54670.37078383444878205No Hit
GTTCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC27300.18515453960950704No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27120.18393374044724656No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23190.15727962540455928No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22700.1539563387961835No Hit
CCACAGAACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCC22100.14988700825531523No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC20620.1398493262545068No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAACAGCTTCCAAGCACAGC19690.13354186391616094No Hit
GTATAATAACTGGCCTCCGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT18170.12323289321262795No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17990.12201209405036746No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17470.11848534091494828No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17250.11699325304996323No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16520.11204223422524016No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15600.10580259406257546No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15300.10376792879214132No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG15200.10308970703532994No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15110.10247930745419968No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG15110.10247930745419968No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14800.1003768200080844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34850.039.7475361
GGTATCA14000.038.8011631
TATCAAC39650.034.7479552
ATCAACG40650.033.629013
TCAACGC40850.033.3733984
CAACGCA41900.032.6169055
AACGCAG42650.032.122856
CGCAGAG44350.030.8150678
ACGCAGA44600.030.7944097
AGAGTAC45150.030.2670111
TGTTAGA6600.029.48407770
GCAGAGT46650.029.4411729
CAGAGTA49050.027.86140410
GCCGTTA1104.3204636E-827.7526615
GTGGTAT7850.027.6798131
CCGCTAT1358.364385E-426.69340570
TGGTATC8050.026.1361852
GAGTACG42850.025.01021612
AGTACGG42650.024.97100313
CGGTACC851.3817113E-424.51056745
Produced by FastQC (version 0.11.5)