FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801679
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGTACTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT76770.42610254101868317No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66970.3717088338155687No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60710.33696346574500785No Hit
GTATTACTGTCATCAGAGTAGTACTTTACCTCGAACTTTTGGCCAGGGGA55800.3097111083605903No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48740.2705254376612038No Hit
GTACTACTCTGATGACAGTAATACGCTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC46620.2587586356948158No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45190.2508215947457899No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44730.24826842073421515No Hit
CAGTAATACGCTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCCGATCCACTGCCA38210.21207995430928595No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36650.2034213641830759No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36450.2023112885258695No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36330.2016452431315456No Hit
GATTTCACCCTCACCATCAATAGCCTGGAACCTGAAGATGCTGCAGCGTATTACTGTCATCAGAGTAGTACTTTA36120.20047966369147888No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35580.19748245941702156No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35290.19587284971407226No Hit
AACTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT34390.19087750925664337No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33370.18521612340489066No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32870.18244093426187463No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGATTCAGGGAGA32430.1799987678160205No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32370.17966574511885858No Hit
GTGTAAGTTACTACCAATGCTCTGACTGGCCCGGCACGTGATGGTGACTTTCTCCTTTGGAGTCACAGACTGAAA31260.17350482522136296No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31030.17222823821557556No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30400.1687314998953754No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28570.15857430763193667No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC27260.15130331207723463No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCGGGGACAGATTTCACCCTCACCATCAATAGCCTGGAAC27240.15119230451151397No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27120.15052625911719014No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT25620.142200691688142No Hit
GCGTATTACTGTCATCAGAGTAGTACTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA25510.1415901500766785No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24750.1373718625792941No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24650.13681682475069087No Hit
GTCCATCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCGGGGACAGATTTCACCCTCACCATCAATAGC24230.1344856658705574No Hit
GGGTTCCAGCCTCCAGGGGTGAAATTGTGCTGACTCAGTCTCCAGAGTTT23770.13193249185898265No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23490.13037838593889367No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTCGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG23410.1299343556760111No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23300.12932381406454757No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23170.1286022648873634No Hit
GTACTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA23110.12826924219020147No Hit
CCCCTGAGATGGACTGGGAAGCATACTTGATGAGGAGCTTTGGAGACTGA23050.12793621949303954No Hit
GTCTCTGGTGGCTCCATCAGTAATTACTACTGGAGCTGGATCCGGCAGCC22660.12577157196148703No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA22510.12493901521858222No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22410.12438397738997903No Hit
GAGATGGACTGGGAAGCATACTTGATGAGGAGCTTTGGAGACTGATCTGGTTTCTGCTGGTACCAGTGTAAGTTA22300.12377343577851549No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21600.11988817097829303No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21430.11894460666966758No Hit
CTCCAAAGCTCCTCATCAAGTATGCTTCCCAGTCCATCTCAGGGGTCCCC21180.11755701209815955No Hit
GTTCAGTGGCAGTGGATCGGGGACAGATTTCACCCTCACCATCAATAGCC21120.11722398940099764No Hit
GTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACGTGCCGGGCCAGTCAGAGCATTGGTAGTAACTTACA21070.11694647048669601No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20910.11605840996093089No Hit
GTAAAGTACTACTCTGATGACAGTAATACGCTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAAT20910.11605840996093089No Hit
GGGTGAAATCTGTCCCCGATCCACTGCCACTGAACCTCGAGGGGACCCCT20310.11272818298931164No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20060.11134058841780362No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19860.1102305127605972No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19750.10961997114913367No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19290.10706679713755891No Hit
GAGCATTGGTAGTAACTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCATCAAGTATGCTTC18830.10451362312598414No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18590.10318153233733644No Hit
TCCCAGTCCATCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCGGG18570.10307052477161582No Hit
GTCTCCAGAGTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACGT18420.10223796802871099No Hit
CACCATCAATAGCCTGGAACCTGAAGATGCTGCAGCGTATTACTGTCATC18410.10218246424585067No Hit
GTAATACGCTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCCGATCCACTGCCA18360.10190494533154908No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18160.10079486967434265No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18140.10068386210862201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT408.461684E-785.8161770
GGTATCA16850.042.1431961
GTATCAA43400.039.300231
TGGTATC5400.039.0091932
GCTATAC350.001453389638.8294263
GTACGTA707.299881E-637.7078369
GTGGTAT5500.037.683241
TAGGGAT12400.035.43377370
GGTACGT701.5726211E-533.71319668
ATCAACG51800.032.7951243
TATCAAC51850.032.5669172
TATCTCG3200.032.1323936
TCAACGC52850.032.0141034
CAACGCA53250.031.7089375
GACGGAC4200.031.57696326
AACGCAG53400.031.5562486
ACGAGAC4500.030.20150422
CGTATGC3900.030.09956641
ATGCCGT3850.029.72704144
ACGCAGA56900.029.6657
Produced by FastQC (version 0.11.5)