FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801679
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGTACTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT72860.4044005619202977No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65060.3611076112892474No Hit
GTACTACTCTGATGACAGTAATACGCTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC51280.2846233985077253No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51170.28401285689626177No Hit
GTATTACTGTCATCAGAGTAGTACTTTACCTCGAACTTTTGGCCAGGGGA48860.27119148305552765No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45170.2507105871800693No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44890.2491564812599803No Hit
CAGTAATACGCTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCCGATCCACTGCCA39740.22057203308691503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39720.2204610255211944No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39480.2191289347325467No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36680.20358787553165686No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35130.19498478918830714No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34260.19015596007945923No Hit
GATTTCACCCTCACCATCAATAGCCTGGAACCTGAAGATGCTGCAGCGTATTACTGTCATCAGAGTAGTACTTTA33780.1874917785021638No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33500.18593767258207483No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33060.1834955061362207No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31550.17511443492431228No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGATTCAGGGAGA31240.17339381765564232No Hit
AACTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT30940.17172870416983269No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30880.17139568147267079No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29100.16151600812353367No Hit
GTGTAAGTTACTACCAATGCTCTGACTGGCCCGGCACGTGATGGTGACTTTCTCCTTTGGAGTCACAGACTGAAA28920.1605169400320479No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28890.16035042868346694No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTT28490.15813027736905408No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27820.1544115239174126No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27710.15380098230594907No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27140.15063726668291078No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26520.1471960321455709No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC26440.1467520018826883No Hit
GGGTGAAATCTGTCCCCGATCCACTGCCACTGAACCTCGAGGGGACCCCT25880.14364379004251035No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA24950.1384819382365005No Hit
GGGTTCCAGCCTCCAGGGGTGAAATTGTGCTGACTCAGTCTCCAGAGTTT24730.13726085501357344No Hit
GCGTATTACTGTCATCAGAGTAGTACTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA23940.13287605616760811No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23900.13265404103616682No Hit
GTACTTTACCTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA23170.1286022648873634No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22740.1262156022243696No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22660.12577157196148703No Hit
GTAATACGCTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCCGATCCACTGCCA22310.12382893956137579No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22220.12332940551563291No Hit
GTCCATCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCGGGGACAGATTTCACCCTCACCATCAATAGC22140.12288537525275034No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCGGGGACAGATTTCACCCTCACCATCAATAGCCTGGAAC22030.12227483364128681No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTCGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG21480.11922212558396916No Hit
GTAAAGTACTACTCTGATGACAGTAATACGCTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAAT21390.11872259153822629No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21030.11672445535525473No Hit
CCCCTGAGATGGACTGGGAAGCATACTTGATGAGGAGCTTTGGAGACTGA20890.11594740239521024No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20820.115558875915188No Hit
GTCTCTGGTGGCTCCATCAGTAATTACTACTGGAGCTGGATCCGGCAGCC20750.11517034943516576No Hit
GAGATGGACTGGGAAGCATACTTGATGAGGAGCTTTGGAGACTGATCTGGTTTCTGCTGGTACCAGTGTAAGTTA20620.11444880025798158No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19660.10912043710339077No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19440.10789935388046373No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19440.10789935388046373No Hit
GTTTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACGTGCCGGGCCAGTCAGAGCATTGGTAGTAACTTACA19350.10739981983472083No Hit
GTTCAGTGGCAGTGGATCGGGGACAGATTTCACCCTCACCATCAATAGCC19230.10673377444039699No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18900.1049021496060064No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18720.10390308151452061No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18400.10212696046299037No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18320.1016829302001078No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18260.10134990750294587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)