FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777390
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGGGTGTGCAGTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT43640.5613655951324302No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC37730.48534197764313924No Hit
CCCTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT27730.3567064150555062No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27120.34885964573766065No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23440.3015217587054117No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23020.2961190650767311No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20840.26807651243262715No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19270.24788072910636874No Hit
ATAATAGGGTGTGCAGTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGA16720.21507866064652237No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16000.20581690014021276No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15540.19989966426118164No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14650.18845109919088232No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14530.18690747243983072No Hit
CAGTATAATAGGGTGTGCAGTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14480.18626429462689253No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13990.17996115206009852No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC13800.1775170763709335No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13570.17455845843141793No Hit
GTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA13540.17417255174365506No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13290.17095666267896423No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12960.16671168911357234No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12940.16645441798839708No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12030.15474858179292247No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11860.15256177722893272No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11810.15191859941599456No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11660.14998906597718006No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGA11640.14973179485200477No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11340.1458727279743758No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10790.13879777203205598No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGAATATTAATAGGTGGTTGGCCTGGTATCAGCAGAAA10660.13712550971841675No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10600.13635369634289096No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA10590.1362250607803033No Hit
GAATATTAATAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGATAGCCCCTAAGCTCCTGATCTATAAGGCGT10490.134938705154427No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10270.13210872277749905No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10260.13198008721491142No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10130.13030782490127218No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCG10060.1294073759631588No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9870.12696330027399375No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9690.12464786014741636No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9470.12181787777048843No Hit
GATCTATAAGGCGTCTAGTTTACAAAATGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA9150.11770153976768417No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8920.1147429218281686No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8640.1111411260757149No Hit
GGCCAAAACTGCACACCCTATTATACTGTTGGCAGTAATAAGTTGCAAAA8610.110755219387952No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAATCTTGATGGGACCCCA8610.110755219387952No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8580.1103693127001891No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8440.10856841482396223No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT8200.10548116132185904No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8060.10368026344563218No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8030.10329435675786927No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7880.10136482331905479No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACT200.00811399150.48563427
GTGGTAT3050.047.356191
TTGCCGA350.001098232541.10319556
GGTACCT751.1441443E-940.26631519
AATGGTA3150.039.37386370
GTATCAA26700.038.6220441
CTTGTAT558.930054E-636.612812
CGGGTAT601.624372E-533.5509417
TATCAAC31150.032.9691622
TTTGCCG450.003812591831.8965355
ATCAACG32350.031.7359773
CAACGCA32550.031.62985
TCAACGC32600.031.5833234
AACGCAG32550.031.5267726
TGTTAGA11650.031.31221270
GACAGGT1101.4078978E-930.5362341
CGGGTAG1101.4260877E-930.50085617
ACGCAGA33700.030.4509357
CGCAGAG34200.030.0057478
CAGAGTA34900.029.59228510
Produced by FastQC (version 0.11.5)