FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572102
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60080.38216349829718427No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT51210.32574222283286963No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC50050.3183635667405804No Hit
GATTATTACTGTGGAACATGGGATGACAGCCTGAAGGGGTACGTCTTCGG47720.30354264545175824No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC39440.25087430713783204No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTCGGACGGTGACCTCGGTCCCA38130.24254151448188477No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC37680.2396791047909105No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37590.23910662285271567No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37220.2367530859956924No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC34880.2218685556026263No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC32640.2076201162519989No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG31720.20176807866156268No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT30650.19496190450746834No Hit
GTCCTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG30260.19248114944195732No Hit
GATTAGGACTATAGATGAGGAGTTTGGGGGCCGTCCCTGGGAGATGCTGG29380.18688354826849657No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28850.18351226574357135No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27940.17772383725737897No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT26740.1700907447481143No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24360.15495177793807274No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG23440.14909974034763648No Hit
GCCTTGGGCTGACCTCGGACGGTGACCTCGGTCCCACTTCCGAAGACGTACCCCTTCAGGCTGTCATCCCATGTT23430.1490361312433926No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG23390.1487816948264171No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22910.14572845782271127No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC22780.1449015394675409No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT22660.14413823021661445No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTCGGACGGTGACCTCGGTC22630.14394740290388283No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA22080.14044890217046985No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG22080.14044890217046985No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC22050.14025807485773825No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG21740.13828619262617822No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGGAACATGGGATGACAGCCTGAAGGGGTACGTCTTCGGAAGTGGGAC21200.1348513009970091No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20680.13154362757632773No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGGTGTTCATTTGCAGATACA20560.1307803183254013No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC20200.12849039057262188No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC20020.1273454266962322No Hit
GAAGTAATACTGTAAACTGGTACCAGCATCTCCCAGGGACGGCCCCCAAA19260.12251113477369788No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18940.12047564343789396No Hit
GCCTGAAGGGGTACGTCTTCGGAAGTGGGACCGAGGTCACCGTCCGAGGTCAGCCCAAGGCCAACCCCACTGTCA18600.11831293389360234No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18600.11831293389360234No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18550.11799488837238296No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17550.11163397794799575No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC17400.11067984138433766No Hit
CTATGTGGACTCTGTGAAGGGCCGGTTCACCATCTCCAGAGACAACGGCAAAGACTCACTGTATCTGCAAATGAA17280.1099165321334112No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA17170.1092168319867286No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16790.10679968602546146No Hit
CCCCTGAGGGCCGCTGATTAGGACTATAGATGAGGAGTTTGGGGGCCGTCCCTGGGAGATGCTGGTACCAGTTTA16710.10629081319151047No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16620.10571833125331562No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16530.10514584931512078No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA16480.1048278037939014No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16240.10330118529204849No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16230.1032375761878046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)