FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362105
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC38800.2848532235033276No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA36840.27046373076965435No Hit
GTCTTGGGCTCAGGCCTGAATCGGTACCTGACCATCAAGAACATCCAGGA35530.26084626368745434No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGAAGTTG33270.24425429757617806No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32770.2405835086135063No Hit
CTCCCAGCCTGTGCTGACTCAGCCACCTTCTGCATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACC30550.22428520561924375No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30000.2202473377603048No Hit
GTAATACACAGCCGTGTCTGCGGCTGTCACAGAGCTCAGCTTCAGGGAGA29960.2199536746432911No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27090.19888334599755525No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26810.19682770417845907No Hit
CACCTGCACCCTGAGCAGCGGCTACAGTAATTATAAAGTGGACTGGTACC24770.18185088521075835No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23940.17575737553272325No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23510.17260049702482555No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22860.16782847137335227No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22850.16775505559409884No Hit
CTGTGGGGCAGACCATGGCAGTGGGAGCAACTTCGCTTGGGTGTTCGGCG22400.16445134552769428No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22030.16173496169531718No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21980.16136788279905No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21830.16026664611024846No Hit
GACCATGGCAGTGGGAGCAACTTCGCTTGGGTGTTCGGCGGAGGGACCAA21300.15637560980981643No Hit
GACTACCACTGTGGGGCAGACCATGGCAGTGGGAGCAACTTCGCTTGGGT21130.15512754156250802No Hit
GCCATGGTCTGCCCCACAGTGGTAGTCACTCTCATCCTCTTCCTGGATGT20830.15292506818490498No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20720.15211749461311722No Hit
GGCCTGAATCGGTACCTGACCATCAAGAACATCCAGGAAGAGGATGAGAG20430.14998843701476758No Hit
CCTGCACCCTGAGCAGCGGCTACAGTAATTATAAAGTGGACTGGTACCAG20350.1494011107807401No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19270.14147220662136914No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19110.14029755415331419No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18940.13904948590600577No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18930.13897607012675234No Hit
GACCAGGGAAGGGCCCCCGGTTTGTGATGCGAGTGGGCACTGGTGGGATTGTGGGATCCAAGGGGGATGGCATCC18850.13838874389272485No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18780.13787483343795082No Hit
GTGGTAGTCACTCTCATCCTCTTCCTGGATGTTCTTGATGGTCAGGTACC18770.13780141765869738No Hit
GTGTATTACTGTGCGAGACGTCGTCTAAATTACGATATTTTGACTGGTTA18290.1342774602545325No Hit
GGATGAGAGTGACTACCACTGTGGGGCAGACCATGGCAGTGGGAGCAACTTCGCTTGGGTGTTCGGCGGAGGGAC18180.13346988668274473No Hit
GTACCAGCAGAGACCAGGGAAGGGCCCCCGGTTTGTGATGCGAGTGGGCA18090.1328091446694638No Hit
GCCCCACAGTGGTAGTCACTCTCATCCTCTTCCTGGATGTTCTTGATGGT17930.13163449220140885No Hit
ATTCAGGCCTGAGCCCAAGACTGAGAAGCGATCAGGGATGCCATCCCCCTTGGATCCCACAATCCCACCAGTGCC17800.1306800870711142No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG17790.13060667129186077No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17560.12891810836903175No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17070.12532073518561343No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17000.1248068247308394No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16930.12429291427606536No Hit
GTACCTGACCATCAAGAACATCCAGGAAGAGGATGAGAGTGACTACCACTGTGGGGCAGACCATGGCAGTGGGAG16710.12267776713248979No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT16550.12150311466443482No Hit
GTGCTGACTCAGCCACCTTCTGCATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGAGCAGCGG15980.11731841524698904No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15840.11629059433744095No Hit
CCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGAGCAGCGGCTACAGT15670.11504252609013256No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGAAGTTGC15210.11166540024447455No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15150.11122490556895394No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14930.10960975842537837No Hit
GCCCAAGACTGAGAAGCGATCAGGGATGCCATCCCCCTTGGATCCCACAA14900.10938951108761805No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14880.1092426795291112No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14720.10806802706105623No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14620.1073338692685219No Hit
CTGGTGGGATTGTGGGATCCAAGGGGGATGGCATCCCTGATCGCTTCTCA14260.10469090121539822No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14190.10417699076062419No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14130.10373649608510357No Hit
CCTCTGCTCCTCACCCTCCTCAGTCTCCTCACAGGGTCCCTCTCCCAGCC13870.10182768582451425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.048.4962961
TACAGAT10950.048.2763770
AGGGGGG4600.043.8505870
TCGTATG4000.040.479440
CGTATGC4100.039.59012641
ATGCCGT4350.037.58909244
TCTCGTA3850.037.41678238
GACCGGA5150.037.35338226
ATCTCGT4000.036.7859337
AGACCGG5300.036.2762125
GCCTATC4350.035.95032533
GTATCAA37700.035.6002461
CACGAGA5450.035.25965521
ACGAGAC5650.035.20620322
CTTATAC5750.035.188011
TATCTCG4200.034.97686836
GAGCCTA5150.034.89625531
ATCTCCG5600.034.3101610
CCTATAG400.002904293433.7206042
AGCCTAT5050.033.61634432
Produced by FastQC (version 0.11.5)