FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362105
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46800.3435858469060755No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC36400.2672334364825032No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34020.24976048102018567No Hit
GTCTTGGGCTCAGGCCTGAATCGGTACCTGACCATCAAGAACATCCAGGA32800.24080375595126663No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA31940.2344899989354712No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGAAGTTG31290.2297179732839979No Hit
CTCCCAGCCTGTGCTGACTCAGCCACCTTCTGCATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACC27060.19866309865979498No Hit
GTAATACACAGCCGTGTCTGCGGCTGTCACAGAGCTCAGCTTCAGGGAGA26500.1945518150216026No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25900.19014686826639648No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25470.1869899897584988No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24680.18119014319747745No Hit
CACCTGCACCCTGAGCAGCGGCTACAGTAATTATAAAGTGGACTGGTACC23620.1734080705966133No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23010.1689297080621538No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22500.16518550332022863No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22460.16489184020321487No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21900.16078055656502252No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20790.15263140506789125No Hit
CTGTGGGGCAGACCATGGCAGTGGGAGCAACTTCGCTTGGGTGTTCGGCG20690.1518972472753569No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20510.15057576324879507No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20400.14976818967700728No Hit
GGCCTGAATCGGTACCTGACCATCAAGAACATCCAGGAAGAGGATGAGAG20280.14888720032596606No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19800.1453632429218012No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19630.1441151746744928No Hit
GACCATGGCAGTGGGAGCAACTTCGCTTGGGTGTTCGGCGGAGGGACCAA19210.14103171194584851No Hit
GTGGTAGTCACTCTCATCCTCTTCCTGGATGTTCTTGATGGTCAGGTACC19110.14029755415331419No Hit
CCTGCACCCTGAGCAGCGGCTACAGTAATTATAAAGTGGACTGGTACCAG19090.1401507225948073No Hit
GACTACCACTGTGGGGCAGACCATGGCAGTGGGAGCAACTTCGCTTGGGT18590.13647993363213556No Hit
GCCATGGTCTGCCCCACAGTGGTAGTCACTCTCATCCTCTTCCTGGATGT17870.13119399752588823No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17790.13060667129186077No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17690.1298725134993264No Hit
GTGCTGACTCAGCCACCTTCTGCATCAGCCTCCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGAGCAGCGG17650.12957885038231268No Hit
GACCAGGGAAGGGCCCCCGGTTTGTGATGCGAGTGGGCACTGGTGGGATTGTGGGATCCAAGGGGGATGGCATCC17350.1273763770047096No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17150.12590806141964092No Hit
GTGTATTACTGTGCGAGACGTCGTCTAAATTACGATATTTTGACTGGTTA16880.12392583537979818No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16830.123558756483531No Hit
GTACCAGCAGAGACCAGGGAAGGGCCCCCGGTTTGTGATGCGAGTGGGCA16720.12275118291174322No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16670.12238410401547604No Hit
GGATGAGAGTGACTACCACTGTGGGGCAGACCATGGCAGTGGGAGCAACTTCGCTTGGGTGTTCGGCGGAGGGAC16270.11944747284533865No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15890.11665767323370813No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC15830.11621717855818751No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG15620.11467544719386538No Hit
GTACCTGACCATCAAGAACATCCAGGAAGAGGATGAGAGTGACTACCACTGTGGGGCAGACCATGGCAGTGGGAG15100.11085782667268676No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14560.10689337459300129No Hit
GCCCCACAGTGGTAGTCACTCTCATCCTCTTCCTGGATGTTCTTGATGGT14510.10652629569673411No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14450.1060858010212135No Hit
CCCTGGGAGCCTCGGTCACACTCACCTGCACCCTGAGCAGCGGCTACAGT14400.10571872212494632No Hit
CTGGTGGGATTGTGGGATCCAAGGGGGATGGCATCCCTGATCGCTTCTCA14360.10542505900793257No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14360.10542505900793257No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14320.10513139589091883No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGAAGTTGC14120.10366308030585014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.043.5729831
TACAGAT9900.040.2425670
GTGGTAT5300.039.4081
GTATCAA41650.035.5073361
GACCGGA850.002753008634.08781470
AGCGATA400.002884816633.76692227
TGGTATC6200.033.6788182
TATCAAC46100.031.9985312
ATCAACG49100.030.1695253
TCAACGC49200.030.0243364
CACCGAA5600.029.7507570
AACGCAG50050.029.579496
GACAGGT2850.029.5504231
CAACGCA50150.029.5205085
CGCAGAG52450.028.2259948
ACGCAGA52800.027.9751667
AGAGTAC53550.027.51951411
GCAGAGT54150.027.4009939
CAGAGTA54500.027.22502310
AGACCGG858.355056E-426.69995569
Produced by FastQC (version 0.11.5)