FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227388
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCCCCGCTCACTTTCGGCGGAGGGA31290.254931610867957No Hit
GTCTAGGACAGACTTCACTCTCACGATCAGCAGACTGGAGCCTGAAGATT23100.18820454493607564No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA22610.18421232731621948No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22190.1807904264991999No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20990.17101356702200118No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATCGTGAGAGTGAAGTCTGTCCTAGACCCACTGCCA18220.14844531639546743No Hit
GTATATTACTGTGCGAAAGATCAGGTTGTTGCGGTCTCGGGCCTTGATGC17860.14551225855230782No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16430.13386150100864602No Hit
GTCCTAGACCCACTGCCACTGAGCCTGTCTGGGATGCCAGTGGCCCTCCT16390.13353560569273937No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16240.13231349825808955No Hit
GTATGGTAGCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16010.13043960019162645No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15790.12864717595414No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA15470.126040013426887No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15220.12400316770247062No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15150.12343285089963402No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15050.12261811260986745No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14330.1167519969235482No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13960.11373746525141194No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13910.11333009610652867No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12990.1058345038406763No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12730.10371618428728326No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12580.1024940768526334No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12510.10192376004979681No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12510.10192376004979681No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12370.10078312644412361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT408.220595E-751.79121839
GGTATCA15400.042.3308831
CGAGACG3200.038.41108323
GTATCAA35300.038.191531
CGTATGC1600.036.79162641
CGCTCAT3350.036.73611528
ACGAGAC3600.035.08588422
TCGTATG1600.034.57880440
AATCGCG501.892188E-434.40407636
TCTCCGA3800.034.11675611
ACGCTCA3650.033.70432327
CACGAGA3950.032.83991221
TATACAC4050.032.0225223
ATCTCCG4050.032.0107810
TATCAAC42200.031.9456422
CCCACGA4100.031.63071419
ATCAACG43450.031.3408013
CTTATAC4050.031.1810951
TCAACGC43750.031.1246264
ATACGGC7750.030.8239256
Produced by FastQC (version 0.11.5)