FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205059
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTAATGGCCAGGGAGG172000.7800244800706013No Hit
GATTATTACTGTGCAACATGGGATGACACCCTGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG146340.6636557117065802No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCT97560.44243714113772015No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC92140.4178572999634023No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCAGGGTGTCATCCCATGTTGCACAGTAATAATCAG83470.37853862413658773No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG82980.3763164613735959No Hit
CCCTGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC77330.350693564208486No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT70640.3203542399545772No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCCTCAGTGTCTGGGACCC69440.31491220869827063No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG67120.3043909482694114No Hit
CCCTCAGGGTGTCATCCCATGTTGCACAGTAATAATCAGCCTCATCCTCG66050.2995384703992047No Hit
GTCCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCAGAGGGTCAC63870.28965211361691456No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC61820.28035531022072424No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA60420.2740062737550333No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCCTCAGTGTCT59900.2716480602106338No Hit
CTAATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC59270.2687909938010729No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT57310.25990234274910556No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT57000.2584964846745597No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG56450.2560022203487526No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG54870.24883687919461567No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG53140.24099128413344043No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG53120.24090058361250197No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC52320.2372725627749643No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC52290.23713651199355665No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA52010.23586670470041846No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC51450.23332709011414207No Hit
GGCCATTAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAACATGGGATGACACCCTGAGGGTGTT48860.22158137265261385No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA48800.22130927108979848No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA48620.22049296640135252No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCAC47780.21668354452193797No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG46490.21083336092140845No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT46400.21042520857718544No Hit
GGATGACACCCTGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT46350.21019845727483938No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC43480.19718293252017294No Hit
CCCCTGAGGGCCGTTGATCATTCCTATGAATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCAGTATA42150.19115134787776655No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG41170.18670702235178288No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA40840.1852104637562986No Hit
CCCTCAGTGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG40590.18407670724456807No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC40150.18208129578392232No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA39950.1811742905745379No Hit
ACATGGGATGACACCCTGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC39900.18094753927219182No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG38970.17672996504855426No Hit
GTATACATAATGACTTCCGATGTTGGAACTACTCCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA38610.1750973556716623No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC38590.17500665515072386No Hit
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA38430.17428105098321633No Hit
CTCTTGTTCTGGGAGTAGTTCCAACATCGGAAGTCATTATGTATACTGGTACCAGCAATTCCCAGGAACGGCCCC37540.17024487780145564No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC37310.16920182181066357No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCAGGGTGTCATCCCATGTTGCACAGTAATAATCAGC36780.16679825800579487No Hit
GTGTCATCCCATGTTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAG36560.165800552275472No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC35630.16158297805183444No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC35630.16158297805183444No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGGAGTAGTTCCAACATCGGAAGTCATTATGTATA35490.16094807440526535No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCTCCATCTCCAGGGACGATGCCAAGAACTCACTGTATCTG34890.15822705877711207No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT34440.15618629705599713No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT33800.153283880385967No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG32930.14933840772514476No Hit
ATGTATACTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCTCATT32680.14820465121341425No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT32360.14675344287839917No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATTAGTGGGCTCCGGTCCGAGGATG32110.14561968636666864No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC31790.14416847803165356No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA31780.14412312777118436No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG31170.14135676188256188No Hit
GTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCAGTATACATAATGACTT31000.1405858074545851No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC30990.1405404571941159No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCAGGGTGT30850.1399055535475468No Hit
GTGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGGAGTAG30820.13976950276613914No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC30120.13659498453329366No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA30040.13623218244953988No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAACATGGGATGACACCCTGAGGGTGTTCGGCGGAGGGACCAAG29890.1355519285425016No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACACTGAGGGTGG29170.13228670978871768No Hit
ATCCCATGTTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC28570.12956569416056443No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG28550.12947499363962597No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT28400.12879473973258768No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC28260.12815983608601855No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATTAGTGGGCTCCGGT28140.1276156329603879No Hit
TCCTCGGACCGGAGCCCACTAATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC28110.12747958217898026No Hit
CCAGTATACATAATGACTTCCGATGTTGGAACTACTCCCAGAACAAGAGA27980.12689002879288036No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC27950.12675397801147273No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGTTG27650.12539347019739608No Hit
AGTCTGTCCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCAGAGG27560.12498531785317309No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC27420.124350414206604No Hit
GTATACTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCTCATTCA27220.12344340899721956No Hit
GCTTGGTCCCTCCGCCGAACACCCTCAGGGTGTCATCCCATGTTGCACAG27010.12249105352736595No Hit
ATGTTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTAATG26990.1224003530064275No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC26810.12158404831798152No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG26490.12013283998296644No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC26110.11840953008513604No Hit
GTGTATTACTGTGCGAGAGACCCCTCACCCATTGCTTCGGAGCCCTGGGGCCAGGGAACCCTGGTCACCGTCTC25870.11732112383387473No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG25850.11723042331293629No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC25730.11668622018730565No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC25650.11632341810355187No Hit
GTCAGGGACCCCTGAGGGCCGTTGATCATTCCTATGAATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGCTGGTA24880.11283144804742186No Hit
GTATATGGTAGTACTACGACTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATT24870.11278609778695263No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTC24860.11274074752648341No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG24840.11265004700554497No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG24410.11069998580536848No Hit
GTGCAACATGGGATGACACCCTGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG24310.11024648320067626No Hit
GCCCCCAAACTCCTCATTCATAGGAATGATCAACGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT24240.10992903137739171No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT24180.10965692981457639No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG23850.1081603712190921No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT23810.10797897017721522No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA23590.10698126444689236No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC23410.10616495975844636No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT23250.10543935559093884No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC23220.10530330480953116No Hit
GATCATTCCTATGAATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGCTGG23170.10507655350718507No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA23140.10494050272577742No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT23050.10453235038155441No Hit
TTATGTATACTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCTCA22990.10426024881873908No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC22830.10353464465123155No Hit
CCTCAGCCTCCCTGGCCATTAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAACATGGGATGACA22620.1025822891813779No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTC22610.1025369389209087No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA22550.10226483735809337No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG22370.10144853266964737No Hit
CCCTCAGGGTGTCATCCCATGTTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTAATGGCCAGGG22340.10131248188823973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG150.002342218869.1009141
CCCGTAT200.0076431951.25801518
AGGGGGG4750.050.02025270
TGGTATC4350.047.925892
GTGGTAT4400.047.3823551
GGTATCA16650.045.775191
TCGTATG5100.045.34406740
TCTCGTA4550.044.6532338
ATGCCGT5050.043.91863344
ACGAGAC5250.042.95910322
CGTATGC5400.042.86815641
CTTATAC5450.042.01631
ATCTCGT4800.041.56642537
ATCTCCG5700.041.36142710
TTATACA5650.040.5280762
GTATGCC5900.040.44912742
CACGAGA5600.039.6639421
TACATCT4950.039.54148534
TCTCCGA5900.039.38022611
TATACAC5950.039.0590443
Produced by FastQC (version 0.11.5)