FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205059
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATGGGATGACACCCTGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG134530.6100970540924302No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC88650.40203005905964423No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG86620.39282395618439236No Hit
GTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCCTCAGTGTCTGGGACCC81500.3696046228241512No Hit
GTCCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCAGAGGGTCAC75960.3444805785242028No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCT73980.33550122695129697No Hit
CCCTGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC68520.31073998473510234No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCCTCAGTGTCT68140.3090166748372719No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTAATGGCCAGGGAGG67040.3040281461856576No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCAGGGTGTCATCCCATGTTGCACAGTAATAATCAG63920.2898788649192607No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT63200.28661364616547674No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA61810.28030995996025504No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG59620.2703782529174957No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG57730.26180705368881285No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57630.2613535510841206No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT56840.2577708805070522No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG55840.2532358544601301No Hit
CTAATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC52560.23836096902622558No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA52480.23799816694247186No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC51330.2327828869885114No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCAC51260.23246543516522689No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC51010.23133167865349635No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC50790.23033397292317345No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT49270.22344073333185188No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG49250.22335003281091342No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG48510.21999411353619108No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC48500.21994876327572185No Hit
GGCCATTAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAACATGGGATGACACCCTGAGGGTGTT48290.21899640780586824No Hit
CTCTTGTTCTGGGAGTAGTTCCAACATCGGAAGTCATTATGTATACTGGTACCAGCAATTCCCAGGAACGGCCCC47600.21586723983349196No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA46220.20960890388873948No Hit
CCCTCAGTGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG45060.20434827367430985No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG45060.20434827367430985No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA44310.20094700413911828No Hit
GGATGACACCCTGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT44310.20094700413911828No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC43270.1962305770503193No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG42450.19251185569184318No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGGAGTAGTTCCAACATCGGAAGTCATTATGTATA41390.18770472808210573No Hit
CCCTCAGGGTGTCATCCCATGTTGCACAGTAATAATCAGCCTCATCCTCG40790.18498371245395248No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39770.18035798588609192No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT39670.17990448328139974No Hit
ATGTATACTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCTCATT39510.1791788791138922No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA38850.1761857619229236No Hit
CCCCTGAGGGCCGTTGATCATTCCTATGAATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCAGTATA38740.17568690905776216No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC38370.174008949420401No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC36410.16512029836843367No Hit
GTATACATAATGACTTCCGATGTTGGAACTACTCCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA36250.16439469420092614No Hit
GTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCAGTATACATAATGACTT36190.16412259263811083No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC35560.1612655262285499No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT35250.15985966815400404No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA34870.15813635825617364No Hit
GTGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGGAGTAG34730.15750145460960455No Hit
ACATGGGATGACACCCTGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC34410.1560502462745895No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA33940.1539187840325361No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT33680.15273967726033635No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC33640.15255827621845947No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC33570.15224082439517492No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC33500.15192337257189037No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC33080.15001866163218308No Hit
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA32830.14888490512045258No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATTAGTGGGCTCCGGTCCGAGGATG32150.14580108740854553No Hit
GTATACTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCTCATTCA31080.14094860953833888No Hit
AGTCTGTCCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCAGAGG31060.14085790901740045No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCTCCATCTCCAGGGACGATGCCAAGAACTCACTGTATCTG30820.13976950276613914No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT30430.13800084260783954No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG29420.13342046630044818No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG29270.13274021239340988No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT29100.13196925796543313No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATTAGTGGGCTCCGGT29080.13187855744449467No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGTTG28310.12838658738836467No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAACATGGGATGACACCCTGAGGGTGTTCGGCGGAGGGACCAAG28250.12811448582554935No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC28100.12743423191851103No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA27790.12602837384396517No Hit
GCCCCCAAACTCCTCATTCATAGGAATGATCAACGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT27730.12575627228114983No Hit
TTATGTATACTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCTCA27300.12380621108097334No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCAGGGTGTCATCCCATGTTGCACAGTAATAATCAGC27300.12380621108097334No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC26990.1224003530064275No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC26730.12122124623422774No Hit
GGGTCACCATCTCTTGTTCTGGGAGTAGTTCCAACATCGGAAGTCATTAT26390.11967933737827424No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCAGGGTGT26310.11931653529452046No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG25710.1165955196663672No Hit
GTGTATTACTGTGCGAGAGACCCCTCACCCATTGCTTCGGAGCCCTGGGGCCAGGGAACCCTGGTCACCGTCTC25520.115733864717452No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTC25500.11564316419651356No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC25160.11410125534056005No Hit
GTGTCATCCCATGTTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAG25110.11387450403821395No Hit
CCAGTATACATAATGACTTCCGATGTTGGAACTACTCCCAGAACAAGAGA24910.11296749882882952No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG24640.11174304179616057No Hit
GTGCAACATGGGATGACACCCTGAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG24600.11156164075428368No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG24580.11147094023334524No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT24560.11138023971240679No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACTCAGCCACCCTCAGTGTCTGGGACCCCCGGGCAGAGGGTCAC24490.11106278788912224No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT24410.11069998580536848No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA24390.11060928528443004No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACACTGAGGGTGG24380.11056393502396082No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC24300.11020113294020704No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC24030.10897667590753807No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC23890.10834177226096899No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG23770.10779756913533833No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTC23650.10725336600970767No Hit
GTATATGGTAGTACTACGACTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATT23310.10571145715375416No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC23110.10480445194436974No Hit
GTCAGGGACCCCTGAGGGCCGTTGATCATTCCTATGAATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGCTGGTA22770.10326254308841623No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC22690.10289974100466245No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC22640.10267298970231635No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG22500.10203808605574727No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG22460.10185668501387038No Hit
GGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT22280.1010403803254244No Hit
CCTCAGCCTCCCTGGCCATTAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAACATGGGATGACA22260.10094967980448595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)