FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070456
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT43030.40197822236504815No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41780.3903009558543275No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38020.35517573819007975No Hit
GTCTTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC36200.3381736381504704No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC32260.30136689410867895No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29660.27707817976637994No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC26820.25054743025402254No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26320.24587652364973434No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25230.2356939472523859No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23720.22158780930743535No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC22760.21261966862720189No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21540.20122265651273846No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG20870.1949636416629922No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG19490.18207193943515662No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18310.1710485998490363No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18090.16899340094314946No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17920.16740529269769147No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16930.1581568976212007No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16090.1503097745259964No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACTAGCAGTGACGTT15700.14666646737465155No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC15640.14610595858213696No Hit
TCTCTGGGCTCCAGGCTGATGATGAGGCTGATTATTATTGTAGTTCATAT15600.1457322860537939No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15120.14124821571367716No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15080.1408745431853341No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC15060.1406877069211626No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14810.13835225361901843No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14730.13760490856233232No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG14570.13611021844896007No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14230.13293400195804403No Hit
GTCAGTCACCATCTCCTGCACTGGAACTAGCAGTGACGTTGGTGGTTTTAACTATGTCTCCTGGTACCGACAGCA13950.13031829425964261No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT13740.12835651348584154No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13210.12340535248529598No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13180.12312509808903868No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13060.1220040805040095No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13020.12163040797566645No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12700.11864102774892195No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12440.11621215631469205No Hit
CCCCTGAGGGCCGCTTAGTGACCTCATAAATAATGAGTTTGGGGGCTTTGCCTGGGTGCTGTCGGTACCAGGAGA12380.11565164752217746No Hit
CTCCTGCACTGGAACTAGCAGTGACGTTGGTGGTTTTAACTATGTCTCCTGGTACCGACAGCACCCAGGCAAAGC12240.11434379367297676No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12060.11266226729543297No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12000.1121017585029184No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG11900.11116757718206073No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT11890.11107415904997497No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG11850.11070048652163189No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11730.10957946893660271No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG11530.10771110629488742No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11520.10761768816280165No Hit
GATTATTATTGTAGTTCATATGCAGGCAGCGGCCAATACGTCTTCGGAACTGGGACCAGGGCCACCGTCTTGGGT11170.10434805353979985No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT11100.1036941266151995No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG11050.10322703595477067No Hit
TTATTGTAGTTCATATGCAGGCAGCGGCCAATACGTCTTCGGAACTGGGACCAGGGCCACCGTCTTGGGTCAGCC10950.10229285463391302No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10820.10107841891679809No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG10750.10042449199219772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3350.049.93285870
GTGGTAT4100.046.419981
TTAGACT306.795679E-445.314744
TGGTATC4300.044.260912
GGTATCA14300.041.1159931
GTATCAA39100.040.0705151
CTCTTAT2500.036.7049371
CACGAGA5700.035.18183521
TCTTATA2750.034.603982
TATCAAC45350.034.4731752
GTCGTAT1500.034.38770739
TATACAC6050.034.266933
ACGAGAC6100.033.99240522
TCGTATG3750.033.98864740
CGCACCG400.002795795733.982885
CGTCGAA400.002797076733.97970614
CGAGACA6150.033.71919623
CCCACGA6050.033.70675319
TTATACA5850.033.6955762
CGTATGC3800.033.59393341
Produced by FastQC (version 0.11.5)