FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070456
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57610.538181858946094No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40990.38292092341955203No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT37900.35405472060505055No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32910.3074390726942537No Hit
GTCTTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC30730.28707391989955683No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC30290.2829635220877832No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC28260.2639996412743728No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT27100.253163137952424No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA25640.23952409066790228No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23740.22177464557160687No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23240.21710373896731858No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23070.2155156307218606No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22510.21028421532505773No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22440.2096302884004574No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC21270.19870036694642282No Hit
GCCTTGGGCTGACCCAAGACGGTGGCCCTGGTCCCAGTTCCGAAGACGTATTGGCCGCTGCCTGCATATGAACTA18910.17665368777418222No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17720.16553693005597614No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16870.1575963888286861No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG16680.15582144431905653No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG16490.154046499809427No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16460.15376624541316972No Hit
GACCCAAGACGGTGGCCCTGGTCCCAGTTCCGAAGACGTATTGGCCGCTGCCTGCATATGAACTACAATAATAAT16390.15311231848856938No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAAGACGGTGGCCCTGGTCCCAGTTCCGAAGACGTATTGGCCG15900.14853483001636686No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15720.1468533036388231No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15210.14208897890244906No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15110.14115479758159138No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14580.13620363658104584No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC14530.135736545920617No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13840.1292906948066992No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13640.12742233216498391No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13570.12676840524038355No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13400.12518029699492553No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12870.12022913599437997No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12860.12013571786229421No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12460.11639899257886359No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACTAGCAGTGACGTT12420.11602532005052053No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12300.11490430246549135No Hit
CCCCTGAGGGCCGCTTAGTGACCTCATAAATAATGAGTTTGGGGGCTTTGCCTGGGTGCTGTCGGTACCAGGAGA12080.11284910355960451No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG11690.10920579640825966No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11640.10873870574783083No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG11540.10780452442697318No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG11390.1064032524456867No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG11180.10444147167188561No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11140.10406779914354256No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11100.1036941266151995No Hit
TCTCTGGGCTCCAGGCTGATGATGAGGCTGATTATTATTGTAGTTCATAT11030.10304019969059913No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC10780.10070474638845502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.042.947871
GTGGTAT3750.041.6623541
GTATCAA38650.039.8075941
GACAGGT2000.033.9638751
TATCAAC45300.033.7294852
TGGTATC4450.033.5728382
TCAACGC46950.032.6673664
ATCAACG47050.032.528853
CAACGCA47450.032.3201145
AACGCAG47850.032.0499346
ACGCAGA49800.030.7949687
CGCAGAG50450.030.3982038
GCAGAGT51750.029.5690149
AGAGTAC51850.029.51336711
CAGAGTA51950.029.45380210
ACAGGTT1401.8189894E-1129.1118931
GCGATTC500.006929572728.2284854
TATTAGA1850.025.6951832
ATAGACG3650.025.1004874
GGATATT8000.025.0483571
Produced by FastQC (version 0.11.5)