FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688260
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT54100.3204482721855638No Hit
GTATTACTGTCAGCAATATAGTCACTCAGGGTACACTTTTGGCCAGGGGA48720.28858114271498464No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT44200.26180801535308545No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT38500.22804544323741602No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT38290.2268015590015756No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38280.2267423264189165No Hit
GGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG37130.21993057941312355No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA36180.214303484060512No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30700.18184402876334213No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30050.17799391089050265No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG29470.17455842109627664No Hit
GGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCCGGCCGTGGCACTCATTTA29280.17343300202575435No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG27900.1652589056188028No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC27790.16460734720955303No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT27560.16324499780839444No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25650.15193157452051226No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTGATTATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG25130.14885148022224065No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24880.14737066565576393No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT24600.14571215334131No Hit
GTGTATTACTGTCAGCAATATAGTCACTCAGGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA22730.13463566038406405No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATAATCAC22420.13279945032163293No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG22400.1326809851563148No Hit
TCTCTAGAGACAACGCCAAGAATTCACTGTATCTGCAAATGGACAGCCTG22390.13262175257365572No Hit
CTCTAGAGACAACGCCAAGAATTCACTGTATCTGCAAATGGACAGCCTGA22350.13238482224301942No Hit
CAACTGTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATCTACGTTGCAGGTGTAGGTCTGGGTGCCCAAGCTG21870.12954165827538414No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG21790.12906779761411158No Hit
CTCCTGCAGGGCCAGTCAGAGTTTTGACACCAACTACTTAGCCTGGTACC21530.12752775046497578No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTCCCACTTGGTGATCAGCACTGAGC21340.12640233139445345No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCTAGAGACAACGCCAAGAATTCACTGTATCTGCAAATGGA21120.12509921457595394No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21090.12492151682797674No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20780.12308530676554559No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC20500.12142679445109165No Hit
GGGTTCCCTGACCCCAGGGGTCGAAGTAGTTGTTCCCGTAATTCCCACCT20380.12071600345918282No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20260.12000521246727401No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20210.11970904955397865No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA19570.11591816426379824No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA19570.11591816426379824No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA19560.11585893168113916No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG19330.11449658227998057No Hit
CTCCAAGGCCTTCCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC19250.11402272161870801No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA18660.11052799924182294No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT18530.10975797566725504No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18500.10958027791927784No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG18390.10892871951002807No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT18130.10738867236089228No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18070.10703327686493788No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGG17970.10644095103834716No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17850.10573016004643834No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGTTTTGTTAGAAGGTGTCCAGTGTGAGGTGCGGCTGGTGGAGT17720.10496013647187045No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT17690.10478243872389324No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA17650.10454550839325696No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17650.10454550839325696No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC17560.10401241514932534No Hit
GGGCTGGAGTGGGTGGCCAAAATAAAGCAAGGTGGAAGTGAGAAAGACTA17540.10389394998400721No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTTTTGACAC17430.10324239157475743No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA17400.10306469382678023No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG17400.10306469382678023No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG17170.10170234442562164No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17170.10170234442562164No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGGTGTCAAAACTCTGA17030.10087308826839468No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGC16980.10057692535509932No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT16970.10051769277244027No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT200.006792616652.809956
TGCGTCG200.00691394252.57401753
GGTATCA11750.042.465521
ACGAGAC4900.039.74400322
GCGGCAT858.589958E-539.3444770
GTATCAA29300.038.2584761
CCCACGA5100.037.5143819
AATCTCG3850.037.5107636
CTTATAC5300.037.4001731
CGTATGC4550.037.221841
ATCTCGT3800.037.13709637
TCGTATG4400.036.87989440
ATGCCGT4700.036.87200544
CGAGCCC5150.036.4856915
GCCGTCT4500.036.2720546
TCTCCGA5200.036.13486511
CCTTTGT19300.036.0419870
CGAGACA5500.036.03269223
TGCCGTC4700.035.4269345
ATCTCCG5450.035.10312310
Produced by FastQC (version 0.11.5)