FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688260
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47650.28224325637046427No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT45840.27152215890917275No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT45310.2683828320282421No Hit
GTATTACTGTCAGCAATATAGTCACTCAGGGTACACTTTTGGCCAGGGGA44400.2629926670062668No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT42340.25079075497849856No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37410.22158909172757751No Hit
GGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG35570.21069029651830878No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT34120.20210157203274376No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32730.19386824304313316No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27550.16318576522573539No Hit
GGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCCGGCCGTGGCACTCATTTA26930.1595133451008731No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26870.1591579496049187No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGGCAG26050.15430087782687502No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG24750.14660064208119603No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTGATTATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG24260.14369824553090166No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23410.13866347600488077No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATAATCAC23090.13676803335979057No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA22930.13582031203724546No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT22530.13345100873088267No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22400.1326809851563148No Hit
GTGTATTACTGTCAGCAATATAGTCACTCAGGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA22380.13256251999099664No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22210.13155556608579247No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22060.13066707734590643No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT21610.12800161112624833No Hit
TCTCTAGAGACAACGCCAAGAATTCACTGTATCTGCAAATGGACAGCCTG21190.12551384265456741No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21030.12456612133202231No Hit
GGGTTCCCTGACCCCAGGGGTCGAAGTAGTTGTTCCCGTAATTCCCACCT20940.1240330280880907No Hit
CTCTAGAGACAACGCCAAGAATTCACTGTATCTGCAAATGGACAGCCTGA20930.12397379550543164No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT20890.12373686517479536No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC20340.12047907312854654No Hit
CTCCTGCAGGGCCAGTCAGAGTTTTGACACCAACTACTTAGCCTGGTACC20190.1195905843886605No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA19570.11591816426379824No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA19560.11585893168113916No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19200.11372655870541268No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19040.11277883738286756No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18850.11165341831234525No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18820.11147572056436805No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT18640.11040953407650479No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG18140.10744790494355136No Hit
CAACTGTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATCTACGTTGCAGGTGTAGGTCTGGGTGCCCAAGCTG17850.10573016004643834No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA17820.10555246229846113No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCTAGAGACAACGCCAAGAATTCACTGTATCTGCAAATGGA17800.10543399713314301No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAACAGCACGTTCCGTGTGGTCAGCGTC17710.10490090388921137No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTCCCACTTGGTGATCAGCACTGAGC17520.10377548481868906No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT17480.10353855448805278No Hit
CTCCAAGGCCTTCCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC17030.10087308826839468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)