FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615771
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTAGCCTGTTGACAATAGTAGATTGCAAAATCCTCAGGCTGCAGGCTATTGATGGTGAGAGTGAAATCTGTC22730.36913073204161934No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14260.2315795969605584No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11720.19033049623967352No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA11330.18399697290064002No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11180.1815610023856271No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAGCAGCTGGTTAGTCTGGTATCAGCAGAAA10880.17668906135560133No Hit
GTATCAACGCAGAGTACGGGAAACTGTTAGCCTGTTGACAATAGTAGATT10760.17474028494359103No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10620.17246671246291234No Hit
GGATATTAGCAGCTGGTTAGTCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATGTATGCTGCATC10320.16759477143288656No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10120.16434681074620272No Hit
CAATAGTAGATTGCAAAATCCTCAGGCTGCAGGCTATTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG9690.15736369526983246No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9590.15573971492649052No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9380.15232935620547247No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8880.14420945448876285No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8560.1390127173900687No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8380.13608955277205323No Hit
CATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGACTAACC8300.1347903684973797No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC7980.12959363139868554No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7950.12910643729568297No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT7740.12569607857466492No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA7640.12407209823132302No Hit
GTGTATTACTGTGCGAGAGATCCGGACATCTCTGGCAGTGGTTGGTTTTACTTTGACTTCTGGGGCCAGGGAACC7290.11838816702962626No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT7270.1180633709609579No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7200.11692658472061855No Hit
CTCCTGATGTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT7040.11432821617127147No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7020.11400342010260307No Hit
GGCTAACAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT6970.11319142993093212No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT6830.11091785745025343No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG6560.10653311052323024No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGACTAACCAGCTGCTA6540.10620831445456184No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG6520.10588351838589345No Hit
CTACTATTGTCAACAGGCTAACAGTTTCCCGTACACTTTTGGCCAGGGGA6390.10377234393954896No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6280.10198596556187285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6250.10149877145887026No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6210.1008491793215335No Hit
GATGTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA6210.1008491793215335No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCAAT350.005160694556.59742470
GCCCGTT200.007766312551.0468337
TTATACA2050.039.851142
GTATCAA24350.039.701121
GCCGTCT1850.039.5452146
GACCGTA1900.039.439826
TCGTATG1850.039.16108740
ATGCCGT1900.038.38330544
GTGGTAT2400.038.2944531
CGTATGC1900.038.2028641
AATGGTA2250.038.1508670
GTACTAG2100.037.38156530
CTTATAC2100.037.2813721
TGCCGTC2000.036.53073545
TATACAC2150.036.4143643
ACTAGAT1800.036.07122832
CGTACTA2100.035.72718429
ATCTCGT1650.035.3524137
CTAGATC1750.035.16917833
CCGTCTT2100.034.87803647
Produced by FastQC (version 0.11.5)