FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685192
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63100.3744380462285603No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT55530.3295173487650072No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51580.3060778831136155No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGATCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA49190.2918955228840393No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG48940.29041201239977404No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA46590.2764670138476803No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44950.26673518507089994No Hit
GAGTTACAGTACCCTGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT43840.26014839852076205No Hit
ACAGTACCCTGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGA43120.25587588832607794No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41300.24507593200062666No Hit
GTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGATCCCGTCAAGGTT38690.229588082544897No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG36280.21528704147657954No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAAT34390.2040717022155339No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGAT34230.20312225550560412No Hit
GCATAGATCAGGACCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATT33790.2005112770532972No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33590.19932446866588494No Hit
GTAGGAGACAGAGTCTCCATCACTTGCCGGGCAAGCCAGACCATTAGCATCTATTTAAATTGGTATCAGCAGAAG33390.1981376602784727No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33120.1965354689554662No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32900.19522997972931275No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT32350.1919662566639291No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT32190.1910168099539993No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC31740.18834649108232177No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCTCCATCACTTGCCGGGCAAGCCAGACCATTAGCAT31710.18816846982420993No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31250.1854388105331618No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT30770.18259047040337242No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30380.1802761940479186No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29430.17463885420771044No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29250.17357072665903944No Hit
GGCCAAAAGTGTACAGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCA28850.171197109884215No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28760.17066304610987948No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA28720.17042568443239703No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28700.17030700359365578No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTGTACAGGGTA28640.16995096107743213No Hit
GTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA28290.1678740463994607No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC27950.1658564721408599No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27630.16395757872100034No Hit
CCTTAGGGGCTTTCCCTGGCTTCTGCTGATACCAATTTAAATAGATGCTA27160.1611685790105816No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26620.15796419636456854No Hit
GTCTATTACTGTGCGAGACGGGGGTCTGGACAGACCAAGTTTATTTACTACTTTGACTACTGGGGCCAGGGAACC26050.15458179246044368No Hit
GTTACAGTACCCTGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAA25940.15392904784736697No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCTGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA25780.15297960113743717No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG25200.14953785681394166No Hit
CCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCTGTA24970.14817302716841763No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC23720.14075547474709113No Hit
CTCCATCACTTGCCGGGCAAGCCAGACCATTAGCATCTATTTAAATTGGTATCAGCAGAAGCCAGGGAAAGCCC23670.14045877265023807No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC23560.13980602803716136No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23500.13944998552093768No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC23370.13867856006911972No Hit
GTCCAGACCCCCGTCTCGCACAGTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAA23200.13766977293981933No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGATCCCA22680.1345840711325475No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22630.13428736903569444No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT21890.12989617800226919No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21870.12977749716352796No Hit
GTCTGCATCTGTAGGAGACAGAGTCTCCATCACTTGCCGGGCAAGCCAGA21790.12930277380856306No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC21720.12888739087296877No Hit
GTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTG21370.12681047619499738No Hit
AAAGTGTACAGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTC21340.12663245493688555No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21250.12609839116255No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21220.12592036990443817No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20990.12455554025891412No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20750.12313137019401943No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20310.1205203917417125No Hit
GTGGGATCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT20020.11879951957996478No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19690.11684128574073459No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19630.11648524322451091No Hit
GTACAGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTCTTCAG19620.1164259028051403No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19570.11612920070828725No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19260.11428964770779829No Hit
AGCCAGACCATTAGCATCTATTTAAATTGGTATCAGCAGAAGCCAGGGAAAGCCCCTAAGGTCCTGATCTATG19230.11411162644968645No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18810.11161932883612075No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18770.11138196715863831No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGATCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA18770.11138196715863831No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTA18690.11090724380367341No Hit
GTCTGCAACCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCTGTACACTTTTGGCCAGGGGA18650.11066988212619096No Hit
CCCCTAAGGTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGATCCCG18520.10989845667437302No Hit
ACCCTGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC18270.10841494619010772No Hit
CCTTGGTCCCCTGGCCAAAAGTGTACAGGGTACTGTAACTCTGTTGACAG18210.10805890367388403No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCTCCATCACTTGCCG17760.10538858480220652No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC17580.10432045725353552No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17150.10176881922059919No Hit
GGTCTGGACAGACCAAGTTTATTTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCAT17150.10176881922059919No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCTCCATCA16950.10058201083318696No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16930.10046332999444572No Hit
GTGTACAGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTCTTC16900.1002853087363339No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)