FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283579
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47070.36670902219497203No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43960.3424798941085823No Hit
GAATTTGTAGACCTTGCATGCAGTAATAAACTCCTACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT35880.27953090538252806No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34260.26690994477161123No Hit
GTAATAAACTCCTACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA34150.2660529659646972No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30140.23481219309446477No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29850.23255288533078214No Hit
GTTTATTACTGCATGCAAGGTCTACAAATTCCGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA28300.22047727486971974No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28220.21985401755560038No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28200.21969820322707057No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27140.2114400438149892No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27020.21050515784381013No Hit
GTAATGGATACAACTGTTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCGTGATCTCTTTGGGTT26420.20583072798791505No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA26110.20341560589570257No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTAGGAGTTTATTACTGCATGCAAGGTCTACAAATT24590.19157371692743494No Hit
CTACAAATTCCGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG24490.19079464528478576No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23370.18206904288711484No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23250.18113415691593582No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTGTTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTC23190.1806667139303463No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23040.17949810646637254No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22620.17622600556724596No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22310.17381088347503348No Hit
GGCCAAAACTGCACGGAATTTGTAGACCTTGCATGCAGTAATAAACTCCT22140.17248646168252987No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21550.16788993899089966No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20570.16025503689293763No Hit
GTCTACAAATTCCGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAA20390.1588527079361691No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20280.157995729129255No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19760.15394455658747924No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT19280.15020501270276312No Hit
GCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG19180.14942594106011395No Hit
GGATACAACTGTTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCGTGATCTCTTTGGGTTCTAAT19080.14864686941746474No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG19070.14856896225319985No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA18740.14599802583245752No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGA18230.1420247604549467No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17730.13812940224170073No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG17480.13618172313507776No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG17450.13594800164228302No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17250.13438985835698466No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17230.13423404402845482No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAAGAGATCACGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA17130.13345497238580561No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16930.13189682910050723No Hit
GTCTCCACTCTCCCTGCCCGTCAGCCCTGGAGAGCCGGCCTCCATCTCCT16920.13181892193624234No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCAGCC16810.13096194312932824No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG16420.1279235637229964No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16010.12472936998813473No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15730.12254796938871702No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15720.12247006222445209No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACTCTCC15500.12075610461062389No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA15440.12028866162503438No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15310.11927586848959043No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15070.11740609654723239No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG15050.11725028221870254No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14850.11569213893340417No Hit
GGATGTAGGAGTTTATTACTGCATGCAAGGTCTACAAATTCCGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGAT14740.11483516012649006No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14690.11444562430516549No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT14420.11234213087001267No Hit
AGCCAGGGCAGTCTCCACAGCTCGTGATCTCTTTGGGTTCTAATCGGGCC14330.1116409663916284No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14200.11062817325618447No Hit
GTTGTATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGCTGACGGG14140.11016073027059496No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13980.10891421564235626No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG13920.10844677265676674No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13680.10657700071440869No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13650.10634327922161395No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13590.10587583623602444No Hit
CTCCAGGGCTGACGGGCAGGGAGAGTGGAGACTGAGTCAGCACAATTTCC13550.10556420757896476No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13400.10439560011499098No Hit
GAGTGGAGGCTGAGGATGTAGGAGTTTATTACTGCATGCAAGGTCTACAAATTCCGTGCAGTTTTGGCCAGGGGA13210.10291536399395752No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13090.1019804780227785No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGCTGACGGGCAGG12960.10096768488733454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT658.547303E-443.2501170
GGTATCA18600.038.2826651
CGTATGC3250.037.17118541
GTATCAA45150.037.1476481
TCGTATG3200.036.58732640
GTGGTAT6050.035.69911
GTACGTA500.00223276335.57701569
GCCGTCT3450.035.36160746
TATAGTG502.1192893E-433.7468035
ATGCCGT3850.033.3868744
GTATGCC3800.032.785742
TATCAAC51900.032.251282
TGCCGTC3900.032.1208845
ATCTCGT2850.031.24184637
ATCAACG53900.031.1797943
TGGTATC7000.030.8542212
TCAACGC54550.030.74644
AACGCAG55100.030.4995546
CAACGCA55500.030.3417225
AATCTCG2950.030.13870436
Produced by FastQC (version 0.11.5)