FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283579
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44280.34497292336505975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37260.29028209405108685No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36390.2835041707600389No Hit
GAATTTGTAGACCTTGCATGCAGTAATAAACTCCTACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT33940.26441691551513385No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31990.2492250184834747No Hit
GTAATAAACTCCTACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA31380.2444726814633147No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30260.2357470790656438No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26430.20590863515217994No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA26200.20411677037408685No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26050.20294816291011306No Hit
GTTTATTACTGCATGCAAGGTCTACAAATTCCGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA25820.20115629813201993No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24200.1885353375211031No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23980.1868213799072749No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23470.18284811452976404No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23000.17918647780931288No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22860.178095777509604No Hit
CTACAAATTCCGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG22380.17435623362488792No Hit
GTAATGGATACAACTGTTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCGTGATCTCTTTGGGTT22290.17365506914650364No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTGTTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTC22140.17248646168252987No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21260.16563063122721702No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21220.16531900257015736No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTAGGAGTTTATTACTGCATGCAAGGTCTACAAATT21200.1651631882416275No Hit
GGCCAAAACTGCACGGAATTTGTAGACCTTGCATGCAGTAATAAACTCCT21060.16407248794191864No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19890.1549573497229232No Hit
GTCTACAAATTCCGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAA19620.15285385628777037No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19190.14950384822437887No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18990.1479457049390805No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG18390.14327127508318538No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC18360.1430375535903906No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18340.14288173926186076No Hit
GCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG18240.1421026676192116No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT17980.14007708134832372No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17670.13766195925611124No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17540.13664916612066727No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16920.13181892193624234No Hit
GGATACAACTGTTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCGTGATCTCTTTGGGTTCTAAT16830.13111775745785806No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG16670.12987124282961937No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16620.12948170700829476No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA16510.12862472820138068No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15840.12340494819563114No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15750.12270378371724686No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGA15690.12223634073165734No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG15590.12145726908900815No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAAGAGATCACGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA15580.12137936192474323No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACTCTCCCTGCCCGTCAGCC15500.12075610461062389No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGCTGACTCAGTCTCCACTCTCC15340.11950958998238519No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15250.11880842550400092No Hit
GTCTCCACTCTCCCTGCCCGTCAGCCCTGGAGAGCCGGCCTCCATCTCCT15210.11849679684694124No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15210.11849679684694124No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG15100.11763981804002716No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA14870.11584795326193402No Hit
GGATGTAGGAGTTTATTACTGCATGCAAGGTCTACAAATTCCGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGAT14110.10992700877780019No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14090.10977119444927036No Hit
GTTGTATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGCTGACGGG14010.10914793713515102No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13910.10836886549250183No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG13680.10657700071440869No Hit
AGCCAGGGCAGTCTCCACAGCTCGTGATCTCTTTGGGTTCTAATCGGGCC13580.10579792907175951No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13380.10423978578646115No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13350.10400606429366638No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13300.10361652847234179No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13280.10346071414381196No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13250.10322699265101719No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13250.10322699265101719No Hit
CTCCAGGGCTGACGGGCAGGGAGAGTGGAGACTGAGTCAGCACAATTTCC12990.10120140638012931No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12910.10057814906600997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)