FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475369
Sequences flagged as poor quality0
Sequence length20-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57161.2024343194444735No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT9110.19164059919767593No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC7680.1615587049218607No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6960.14641257633543625No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA6950.1462022134384026No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6580.13841878624815668No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG6470.1361047943807863No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6300.13252862513121386No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC6270.13189753644011284No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA5960.12537628663206898No Hit
GAGTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAC5780.12158975448546287No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC5740.12074830289732817No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG5070.1066539887960721No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG4860.102236367958365No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG4800.10097419057616293No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC4770.10034310188506192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATATA150.003052389564.6275564
AGTCATT551.7750557E-459.45309470
CTTTAGG200.00952071748.4808713
CGGACCT200.00952863948.4706654
TAACACT308.707717E-443.0850374
GTGGTAT806.91216E-1140.404981
GTATCAA4500.038.0704651
TGGTATC851.3278623E-1038.0242082
GACAGGT757.1175236E-834.4789161
ATTCAGA450.00310255733.26378354
TATCAAC5150.033.2619552
ATCAACG5200.032.3137783
TCAACGC5250.032.0060234
CAACGCA5350.031.4044785
AACGCAG5450.030.8282496
GGTATCA2050.029.958811
CGCAGAG5650.029.736988
TTCACAG2202.6013004E-729.72654770
GACAGGA554.7667834E-429.3854371
ACGCAGA5800.028.9679227
Produced by FastQC (version 0.11.5)