FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547153
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT39950.7301431226731828No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18940.34615546291439503No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17500.3198374129356871No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13380.2445385477188282No Hit
CCTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA13260.2423453768872692No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12990.23741074251626146No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12540.2291863518979152No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11550.21109269253755347No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11540.21090992830159025No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA11320.20688911511039873No Hit
GGCCAAAGGTGTACCCCGGAGGCCAGCTGCTACGTTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGG11070.2023200092113175No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10570.193181797413155No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10490.191719683525449No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT10310.1884299272781105No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10240.18715057762636775No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9350.17088456062563853No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGCTT9300.16997073944582228No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC9160.1674120401423368No Hit
GTTTATTACTGTCAACAACGTAGCAGCTGGCCTCCGGGGTACACCTTTGGCCAGGGGACCAGGCTGGAGATCAAA8620.1575427714003213No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8490.15516683633279904No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8420.15388748668105628No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8350.15260813702931356No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8240.1505977304337178No Hit
CTCCTGCAGGGCCAGTCAGACTATTAGCTTCTACTTAGCCTGGTACCAACACAAACCTGGCCAGGCTCCCAGGCT8170.14931838078197507No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAACGTAGCAGC8110.14822179536619556No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGCTTCTACTTAGCCTGGTA8060.1473079741863793No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAACGTAGCAGCTGG7950.14529756759078355No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7840.14328716099518782No Hit
GCCATAAGGGCCACTGGCGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGG7790.14237333981537156No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7790.14237333981537156No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGTGGCCCTTAT7760.14182504710748184No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA7520.13743870544436382No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7250.13250407107335607No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7070.12921431482601758No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7030.12848325788216458No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6700.12245203809537736No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6680.12208650962345084No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAGGTGTACCCCGGAGG6670.12190374538748759No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6510.1189795176120756No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6480.11843122490418584No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6430.1175174037243696No Hit
ACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCAGCCATAAGGGCCACTGGCGTCCCAGCCAGGTT6310.1153242328928106No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAGGTG6290.11495870442088411No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6280.11477594018492086No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6080.11112065546565586No Hit
GGTGTACCCCGGAGGCCAGCTGCTACGTTGTTGACAGTAATAAACTGCAA6070.1109378912296926No Hit
GGGTACACCTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG6060.11075512699372937No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6000.10965854157794987No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5880.10746537074639087No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5780.10563772838675836No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5760.10527219991483187No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCA5650.10326179331923613No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5640.10307902908327288No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5580.10198244366749337No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5570.10179967943153012No Hit
GTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCAGCCATAAGGGCCACTGGCGTCCCAGC5560.10161691519556687No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT5530.10106862248767713No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5480.10015480130786088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA353.178211E-759.4229341
CGTTAGC200.006355791453.6995751
ACGTGTT200.00668882553.0107147
AATATCG453.3740434E-852.92695236
ATCGTAT601.0004442E-1051.61622639
TATCGTA552.763045E-949.82578738
CTTATAC10150.045.8492741
TATACAC10400.045.3863683
TTATACA10550.044.425992
ATACACT307.580159E-444.3169144
TCGTATG10250.043.78384840
ATGCCGT10100.043.69322244
CTTGAAA9900.043.60180757
ATATCGT551.6070771E-743.40539637
TAATATC551.6685044E-743.1987835
GCCGTCT10050.042.77202246
CGTATGC10150.042.6888941
CGTCTTC10100.042.4582548
TGAAAAA11550.042.3881559
GTATGCC10300.042.22417442
Produced by FastQC (version 0.11.5)