FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552580
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC25660.46436715045785226No Hit
GCCTTGGGCTGACCGAGGACGGTCAACTTGGTTCCTCCGCCGAACAGCCAACCACTCAGGCTGCTGTCATAGGA21370.3867313330196533No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17700.3203156104093525No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15340.27760686235477217No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15200.2750732925549242No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGC13720.24828984038510263No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13340.2414130080712295No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAACTTGGTTCCTC12940.23417423721452096No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12880.2330884215860147No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG12670.2292880668862427No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12570.22747837417206557No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12480.22584965072930618No Hit
GGTTATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAGACTCCTCATCCATACTAACATCGATCGG12230.22132541894386332No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12220.22114444967244562No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12180.22042057258677478No Hit
TACTAACATCGATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCGCCTCAGCCTCCCTGGC11650.21082920120163595No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTGGCTGTTCGG10640.19255130478844693No Hit
GTCCTATGACAGCAGCCTGAGTGGTTGGCTGTTCGGCGGAGGAACCAAGTTGACCGTCCTCGGTCAGCCCAAGG10620.1921893662456115No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10510.19019870426001664No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10330.18694125737449782No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10290.18621738028882695No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10200.18458865684606754No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10100.1827789641318904No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9950.1800644250606247No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9640.1744543776466756No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9030.16341525209019508No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8680.15708132759057514No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8530.15436678851930943No Hit
ATGTTAGTATGGATGAGGAGTCTGGGGGCTGTTCCTGGAAGCTGCTGGTA8510.154004849976474No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8430.1525570958051323No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC8400.15201418799087915No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT8150.1474899562054363No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCGCCTCAG8000.1447754171341706No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT7870.14242281660574035No Hit
GTGATGGCCAGGGAGGCTGAGGCGCCAGACTTGGAGCCAGAGAATCGGTC7860.14224184733432263No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTT7820.1415179702486518No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7690.1391653697202215No Hit
GCCCCCAGACTCCTCATCCATACTAACATCGATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT7670.13880343117738608No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT7590.13735567700604437No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7570.13699373846320895No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7450.1348221072061964No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAACTTGGTTCCTCCGCCGAACAG7430.13446016866336094No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT7360.13319338376343695No Hit
GCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAGACTCCTCAT7340.13283144522060153No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAACTTGGTT7230.1308407832350067No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC7160.1295739983350827No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGCTGTACACTGGTACCAG7100.12848818270657641No Hit
GTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAGACTCCTCATCCA6980.12631655144956386No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATG6910.12504976654963987No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6740.12197328893553874No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6690.12106844257845017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.12088747330703246No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG6540.11835390350718449No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6460.11690614933584279No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6440.11654421079300735No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6400.11582033370733649No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6300.11401064099315936No Hit
AGGTTATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAGAC6240.11292482536465309No Hit
ATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAGACTCCTCATCCATACTAACATCGATCGGCCCT6190.11201997900756452No Hit
GACCGAGGACGGTCAACTTGGTTCCTCCGCCGAACAGCCAACCACTCAGG6100.11039125556480509No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6100.11039125556480509No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5960.1078576857649571No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA5860.10604799305077997No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGCGCC5860.10604799305077997No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC5830.10550508523652682No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT5800.1049621774222737No Hit
GCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACA5730.10369539252234969No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC5720.10351442325093199No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC5690.10297151543667885No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC5580.10098085345108401No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC5570.10079988417966629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1200.064.0585870
GGTATCA7300.047.0873641
GTGGTAT1600.044.668891
GATGGGT658.7816163E-443.0043670
AATGGTA2400.040.7645570
GTATCAA20850.040.317791
GAGTCGG350.001441737438.88832512
TGGTATC1850.038.629062
GCTTGTG451.0844316E-437.8149341
ACCGTAC1900.037.66704627
GACCGTA1900.037.65339726
CGTATGC1800.036.4420141
TATCAAC23250.035.1281172
GACAGGT1750.035.0058251
CGTACTA1950.034.99477829
GTAGTTT1001.7240102E-434.94104470
ATCTCCG2050.034.85721610
ATGCCGT1900.034.67682644
TCGTATG1800.034.4765340
TCTCGTA1700.034.3819638
Produced by FastQC (version 0.11.5)