FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552580
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC24280.43939339100220787No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18770.3396793224510478No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17610.3186868869665931No Hit
GCCTTGGGCTGACCGAGGACGGTCAACTTGGTTCCTCCGCCGAACAGCCAACCACTCAGGCTGCTGTCATAGGA16790.3038474067103406No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13580.2457562705852546No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12930.23399326794310327No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12870.23290745231459697No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12350.2234970502008759No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12300.2225922038437873No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGC12160.22005863404393933No Hit
GGTTATGCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAGACTCCTCATCCATACTAACATCGATCGG11610.2101053241159651No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11560.2092004777588765No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAACTTGGTTCCTC10730.19418002823120636No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG10390.18802707300300409No Hit
GTCCTATGACAGCAGCCTGAGTGGTTGGCTGTTCGGCGGAGGAACCAAGTTGACCGTCCTCGGTCAGCCCAAGG10380.1878461037315864No Hit
TACTAACATCGATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCGCCTCAGCCTCCCTGGC10350.18730319591733324No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10210.18476962611748526No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG10150.18368381048897897No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10140.18350284121756125No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10120.18314090267472583No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTGGCTGTTCGG9690.17535922400376416No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG9320.16866336096130877No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9320.16866336096130877No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9020.1632342828187774No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8670.1569003583191574No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAACTTGGTTCCTCCGCCGAACAG8440.15273806507655No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8100.14658510984834774No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7990.1445944478627529No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7920.14332766296282892No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7830.14169893952006948No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT7670.13880343117738608No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC7650.13844149263455066No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT7590.13735567700604437No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTT7510.13590792283470265No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7500.13572695356328496No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGCTGTACACTGGTACCAG7230.1308407832350067No Hit
GTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAGACTCCTCATCCA7170.1297549676065004No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7000.12667848999239928No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6890.12468782800680443No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC6860.12414492019255131No Hit
GCTGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAGACTCCTCAT6850.12396395092113359No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC6780.1226971660212096No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT6710.12143038112128561No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6670.12070650403561475No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6660.12052553476419704No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCGCCTCAG6620.11980165767852619No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6500.11763002642151363No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6470.11708711860726048No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATG6340.11473451807883023No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6330.1145535488074125No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6320.11437257953599478No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAACTTGGTT6230.11274385609323537No Hit
ATGTTAGTATGGATGAGGAGTCTGGGGGCTGTTCCTGGAAGCTGCTGGTA6230.11274385609323537No Hit
GTGATGGCCAGGGAGGCTGAGGCGCCAGACTTGGAGCCAGAGAATCGGTC6210.11238191755039995No Hit
GCCCCCAGACTCCTCATCCATACTAACATCGATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT6210.11238191755039995No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG6160.11147707119331138No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC5990.10840059357921025No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC5980.10821962430779253No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT5950.10767671649353941No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5930.10731477795070396No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC5580.10098085345108401No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA5540.10025697636541316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)