FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291618
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTATTACTGTGCAGCATGGGATGGCAGCCTGAATGGTTGGGTGTTCGG21030.7211489002736456No Hit
GTAATAATGAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGC19770.6779416908421291No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC11590.3974377438978389No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11220.38474991255683805No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9930.3405139600436187No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACCCAACCATTCAGGCTGCCATCCCATG9610.32954070050545575No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8840.3031362947417512No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8670.2973067506121021No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC8390.28770514851620954No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT7920.27158817356953274No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG7740.2654157150793161No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG7460.25581411298342355No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7390.2534137124594504No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC7290.24998456885377446No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7120.24415502472412542No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC6990.2396971380367467No Hit
GCATGGGATGGCAGCCTGAATGGTTGGGTGTTCGGCGGAGGGACCGAGCTGACCGTCCTAGGTCAGCCCAAGG6960.23866839495504394No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT6950.2383254805944763No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6840.2345534226282328No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTC6810.23352467954653006No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC6690.22940970721971893No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6360.21809353332098844No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG6290.21569313279701527No Hit
GACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACCCAACCATTCAGG6280.21535021843644767No Hit
CCCCTGAGGGCCGCTGATAATTAGTATAGATGACGAGTTTGGGGGCCGTTCCTGGGAGATGCTGGTACCAGTTTA6270.2150073040758801No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG6130.2102065030279338No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA6100.20917775994623106No Hit
TTATTACTGTGCAGCATGGGATGGCAGCCTGAATGGTTGGGTGTTCGGCG5920.2030053014560144No Hit
ATCCCATGCTGCACAGTAATAATGAGCCTCATCCTCAGACTGGAGCCCAC5550.1903174701150135No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC5550.1903174701150135No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5550.1903174701150135No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG5530.18963164139387828No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGCCCGAGGTGCCAGACTTGGAGGCAGAGAATCGGTCAGGGACC5520.1892887270333107No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC5440.18654541214876996No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC5390.184830840345932No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5370.18414501162479682No Hit
GTCTGAGGATGAGGCTCATTATTACTGTGCAGCATGGGATGGCAGCCTGAATGGTTGGGTGTTCGGCGGAGGGAC5370.18414501162479682No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5090.17454340952890424No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC4960.17008552284152556No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA4900.16802803667812002No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC4870.16699929359641724No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA4850.16631346487528204No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCGGGCTCCCTGGCCATCAGTGGGCTCCAGT4630.158769348942795No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT4550.1560260340582543No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA4540.1556831196976867No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTCATTATTACTGTGCAG4430.1519110617314432No Hit
GTCTGGCACCTCGGGCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG4390.15053940428917284No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC4370.14985357556803763No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT4260.1460815176017941No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC4250.14573860324122653No Hit
CTGATGGCCAGGGAGCCCGAGGTGCCAGACTTGGAGGCAGAGAATCGGTC4220.14470986015952375No Hit
GTGCCAGACTTGGAGGCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG4160.14265237399611821No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACAC4100.14059488783271265No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG4050.1388803160298747No Hit
CAGTAATAATGAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA4000.13716574422703673No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG4000.13716574422703673No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT3940.1351082580636312No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG3810.1306503713762525No Hit
ATACTAATTATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAGTCTGGCACCTCGGGCTCCCTGG3790.1299645426551173No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC3780.1296216282945497No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT3730.12790705649171177No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTATGTAAACTGGTACCAGCATCTCCCAGGAACGGCCCC3690.1265353990494414No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT3690.1265353990494414No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTC3670.1258495703283062No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA3670.1258495703283062No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC3670.1258495703283062No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG3620.12413499852546823No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC3620.12413499852546823No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC3530.12104876928035993No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC3530.12104876928035993No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC3530.12104876928035993No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC3520.12070585491979233No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG3470.11899128311695438No Hit
GTGCAGCATGGGATGGCAGCCTGAATGGTTGGGTGTTCGGCGGAGGGACC3450.11830545439581919No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG3430.117619625674684No Hit
ACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACACCCAACCATTCAGGC3420.1172767113141164No Hit
GTAATACACAGCCGTGTCTTCACCTCTCAGGCTGATCATTTGGAGATACA3410.11693379695354883No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA3400.11659088259298124No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG3380.11590505387184605No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC3290.11281882462673772No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC3250.11144716718446736No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC3250.11144716718446736No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC3180.10904676666049422No Hit
ATGCTGCACAGTAATAATGAGCCTCATCCTCAGACTGGAGCCCACTGATG3010.10321722253084514No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG3000.10287430817027755No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC2990.10253139380970996No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG2950.10115973636743959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)