FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3706291
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGAGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGTCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC198990.5368979392066084No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCA188460.5084867863856346No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC185710.5010669696470137No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG117320.31654287264545605No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG116920.3154636265743839No Hit
CTCGTGGACCCCTGCCCGTTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGA112610.3038347501585817No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC111260.3001922946687133No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA111020.29954474702607No Hit
CTCCTATAGTGGTGCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCC109320.2949579512240134No Hit
GTCCACGAGTGTCTGTTGTTTGTAGCATAAATCAGTGTCCTGGGGGCTTG108640.2931232329031908No Hit
CCACTATAGGAGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGTCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG99450.2683275544203086No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGACTCCA94210.2541894308892637No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG93780.25302924136286115No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC90250.2435048947856496No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT85880.23171413145918657No Hit
TCCTCAGTCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAAACGGGCAGGGGTC84200.2271812979606836No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG83440.22513073042564657No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT76800.2072152456458492No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT76320.2059201503605626No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA75430.20351882785242711No Hit
GTAATAGTCAGCCTCATCCTCAGTCTGCGCACCCGAAAGGGTCAGGGCAG74940.20219675141536375No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC72850.1965576906940119No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG72420.19539750116760934No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG70720.19081070536555278No Hit
GACTGAGGATGAGGCTGACTATTACTGCTTGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGGGGGACCAAG70230.18948862892848944No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC69370.18716824987568434No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCC67730.1827433409842886No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG67620.18244654831474377No Hit
CTCCAGCACTGGAGCTGTCGCCAGTGGTCATTATCCCTACTGGTTCCAGG66310.17891201743198254No Hit
CCTATAGTGGTGCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTG65630.1770772991111599No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA65390.17642975146851664No Hit
GACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCAAGCACCACTATAGGAGAGCAAGCAGTAATAGTCAG65030.17545843000455172No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT65010.17540446770099813No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC64640.1744061650852564No Hit
GACTATTACTGCTTGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAG63160.1704129546222895No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT62820.1694955954618782No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT62460.16852427399791328No Hit
ACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCAAGCACCACTATAGGAGAGCAAGCAGTAATAGTCAGC62080.16749899023039475No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA61010.1646120069902768No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG60540.16334389285676706No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC58890.1588920028135945No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA58310.1573270960105399No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC57910.1562478499394678No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAAACGGGCAGGGGTCCAC52180.1407876499713595No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT51330.13849425207033123No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT50640.1366325525977318No Hit
GATTTATGCTACAAACAACAGACACTCGTGGACCCCTGCCCGTTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC47070.12700028141341302No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG46710.1260289599494481No Hit
GCACTGGAGCTGTCGCCAGTGGTCATTATCCCTACTGGTTCCAGGTGAAG46020.12416726047684869No Hit
CCCAAGCACCACTATAGGAGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGTCTGCGCACCCGAAAGGGTCAGGG45800.12357367513775902No Hit
GTCCCCAGGAGGGACAGTCACTCTCACCTGTGACTCCAGCACTGGAGCTG45440.12260235367379409No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA45240.12206273063825802No Hit
AAGCTGCCCTGACCCTTTCGGGTGCGCAGACTGAGGATGAGGCTGACTATTACTGCTTGCTCTCCTATAGTGGTG45070.12160405105805239No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT44740.12071367304941788No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG44620.12038989922809623No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC43670.11782668980929992No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC43390.11707121755954943No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAACAC42790.11545234845294122No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC41320.11148611914175113No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA41210.11118932647220632No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA40070.10811347516965074No Hit
CTCCTATAGTGGTGCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCT40030.10800555056254353No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGACTCCAGC39940.1077627201965523No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT39900.10765479558944507No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT39530.10665649297370336No Hit
CCCCAGGAGGGACAGTCACTCTCACCTGTGACTCCAGCACTGGAGCTGTC39210.10579309611684566No Hit
CTATTACTGCTTGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAGCC39060.1053883788401936No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC38950.10509158617064877No Hit
CAGTAATAGTCAGCCTCATCCTCAGTCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC38660.10430913276912147No Hit
GGTGAAGCCTGGCCAAGCCCCCAGGACACTGATTTATGCTACAAACAACAGACACTCGTGGACCCCTGCCCGTTT38290.10331083015337975No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC37370.10082856418991384No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA37190.1003429034579314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)