FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3706291
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGAGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGTCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC177370.47856468906515975No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC169820.4581939194736733No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCA165620.4468618357274159No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG119700.3229643867683353No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA116810.31516683390483907No Hit
CTCGTGGACCCCTGCCCGTTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGA110390.2978449344641314No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC109090.29433738473314697No Hit
CTCCTATAGTGGTGCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCC108020.29145040149302903No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG100990.27248265179393627No Hit
GTCCACGAGTGTCTGTTGTTTGTAGCATAAATCAGTGTCCTGGGGGCTTG96530.26044905810148206No Hit
CCACTATAGGAGAGCAAGCAGTAATAGTCAGCCTCATCCTCAGTCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG90090.24307319635722074No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG82940.2237816728368064No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGACTCCA82340.2221628037301982No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG81600.22016619849871474No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT77950.21031807810018155No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA77930.21026411579662796No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC77310.20859128438646615No Hit
TCCTCAGTCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAAACGGGCAGGGGTC75530.20378863937019517No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT74220.20025410848743394No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG73630.19866222053260252No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT69500.18751900484878278No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC68540.18492881427820967No Hit
GACTGAGGATGAGGCTGACTATTACTGCTTGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGGGGGACCAAG67620.18244654831474377No Hit
GTAATAGTCAGCCTCATCCTCAGTCTGCGCACCCGAAAGGGTCAGGGCAG67430.1819339064309845No Hit
CTCCAGCACTGGAGCTGTCGCCAGTGGTCATTATCCCTACTGGTTCCAGG64860.17499975042434607No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC64120.1730031451928626No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC64010.17270635252331779No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT63380.17100653996137916No Hit
CCTATAGTGGTGCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTG63260.17068276614005753No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT62750.16930672739944055No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG61110.16488181850804484No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA60210.16245351484813253No Hit
GACTATTACTGCTTGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAG59500.1605378530719795No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC58910.15894596511714812No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG58030.15657162376078942No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG57510.15516860386839565No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCC56060.15125633686075918No Hit
GACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCAAGCACCACTATAGGAGAGCAAGCAGTAATAGTCAG55580.1499612415754726No Hit
ACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCCAAGCACCACTATAGGAGAGCAAGCAGTAATAGTCAGC54950.148261429013534No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAACAC53130.14335085939015582No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA52520.1417050091317708No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG51290.13838632746322402No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT50970.1375229306063663No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC50100.13517557040178443No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAAACGGGCAGGGGTCCAC49220.13280122904542574No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA48890.13191085103679123No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC48440.13069669920683508No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG47720.12875405627890527No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT47260.1275129232971723No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT46590.12570518612812648No Hit
AAGCTGCCCTGACCCTTTCGGGTGCGCAGACTGAGGATGAGGCTGACTATTACTGCTTGCTCTCCTATAGTGGTG45850.12370858089664302No Hit
GATTTATGCTACAAACAACAGACACTCGTGGACCCCTGCCCGTTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC45750.12343876937887499No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45390.12246744791491009No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA43460.11726008562198703No Hit
GTCCCCAGGAGGGACAGTCACTCTCACCTGTGACTCCAGCACTGGAGCTG42760.11537140499761082No Hit
GCACTGGAGCTGTCGCCAGTGGTCATTATCCCTACTGGTTCCAGGTGAAG42200.11386046049810983No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC41190.1111353641686527No Hit
CTCCTATAGTGGTGCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCT39880.10760083328589147No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT39580.10679139873258738No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT39360.10619781339349771No Hit
GGTGAAGCCTGGCCAAGCCCCCAGGACACTGATTTATGCTACAAACAACAGACACTCGTGGACCCCTGCCCGTTT38590.10412026470668384No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC38350.10347271706404057No Hit
CTATTACTGCTTGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAGCC37460.10107139455590508No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC37120.10015403539549377No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAACACC37110.10012705424371697No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT37090.10007309194016337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT1450.053.9898970
TCCGAGA22500.042.3847970
GTGGTAT7250.041.7677231
TGTGTCG44950.039.10704470
AATGGTA20350.038.81917670
TGGTATC7800.038.8084072
GTATCAA69700.035.6351781
TAGGTGG21400.034.4570245
TATCAAC74200.033.0034262
ATAGGTG22350.032.9932984
GTCATAG22950.032.3193471
GTCAGGT22850.032.1200311
TCAGGTC23000.032.0626112
ATGGCAA11250.031.63043870
ATCAACG77350.031.3794653
GGATGGG44950.031.1906470
GTACGTA1707.2759576E-1231.07830269
AGGATGG28350.031.0600369
AACGCAG79750.030.6776356
CAACGCA79100.030.6298475
Produced by FastQC (version 0.11.5)