FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060352
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61930.30057970676855217No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTGTGATTGGTGGTATCATAAGACTGA56150.27252624794209923No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAGTGGCAGCATTGCCAG53110.25777148759046997No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG52510.2548593638368589No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC50770.24641420495138694No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAGTGGCAGCATTGCCAGCAACTATGTG49620.24083263442363245No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT47780.2319021215792253No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTGTGATTGGTGGTATCATAAGACTGACAGTAGTAGT44280.21491473301649427No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA41030.19914072935110116No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38740.18802612369148575No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC36620.17773661976206007No Hit
GACTACTACTGTCAGTCTTATGATACCACCAATCACAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT36350.1764261640729351No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG33570.1629333240145373No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG32810.15924463392663002No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32040.15550740844282918No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31870.15468230671263938No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC31510.15293503246047277No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31220.15152750597956077No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA31210.15147897058366727No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30960.15026558568632933No Hit
CCCTGTGATTGGTGGTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCC30820.1495860901438201No Hit
GTCTTATGATACCACCAATCACAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC30650.1487609884136303No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30140.1462856832230609No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAC29470.14303381169819526No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29110.14128653744602865No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28950.14050997111173236No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28820.13987901096511665No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA28080.13628739166899637No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27210.13206481222626038No Hit
ATCACAGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC27110.1315794582673252No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26530.12876440530550118No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26510.12866733451371418No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGA26180.1270656664492281No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25990.12614349392725127No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG25780.12512425061348742No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGC25140.1220179852763023No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAACCGATCAGGGACCCCAGA25060.12162970210915416No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24970.12119288354611252No Hit
CTACTACTGTCAGTCTTATGATACCACCAATCACAGGGTGTTCGGCGGAG24480.11881464914733017No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24450.11866904295964961No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24010.11653348554033485No Hit
GTCTTTGGTTATCCTCATAGATCACAGTGGTGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCACATAGTTG23630.1146891404963812No Hit
GTTCTCTGGCTCCATCGACAGCTCCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAAGACTGAGGACGAGG23020.11172848134687666No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22990.11158287515919608No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22420.10881635759326562No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCC22260.10803979125896934No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22240.10794272046718231No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22220.10784564967539527No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22060.10706908334109899No Hit
GAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC21590.10478791973410367No Hit
GTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC21460.10415695958748795No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21210.10294357469015003No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21110.10245822073121486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG15150.041.0572970
CTTACGT350.001501693938.57222
GTATCAA49000.035.3477631
ATCAACG56550.030.3100763
TCAACGC56850.030.2622184
GGATGGG14600.030.0733970
AACGCAG57450.029.9454366
CAACGCA58250.029.4762575
TATCAAC58550.029.283252
CGCAGAG61350.028.1517858
AGAGTAC61950.027.76954811
CAGAGTA62800.027.44740110
ACGCGTG500.00859578727.0103781
GACAGGT3750.027.0103761
CGACGTC500.00863455326.98546810
ATCGCTA500.00863455326.98546810
GTGAGTT14550.026.89742951
TTAACGG1655.456968E-1226.86410535
CGGCATT14250.026.39182560
AATGGTA5850.026.26923670
Produced by FastQC (version 0.11.5)