FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821717
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTACACTCCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT61930.3399540104198402No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54690.300211284189586No Hit
GTGTAACTCTGTTGACAGTAGTAAGCTGCAGAATCTTCAGGTTGCAGACTGGTGATGGTGAGTGTGAAATCTGTC52950.2906598555099392No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49280.270514026053443No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG43590.2392797564056327No Hit
CCATAGATCAGGACCTGAGGGGCTTTCCCTGGTTTCTGCTCATACCAATT42670.2342295757244402No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41280.22659941143437756No Hit
GATCTATGGTGCATCCAATTTGCAAGGTGGGGTCCCACCAAGGTTCAGCG38840.2132054539755626No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37210.20425785124692802No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34650.1902051745688271No Hit
GTAGTAAGCTGCAGAATCTTCAGGTTGCAGACTGGTGATGGTGAGTGTGAAATCTGTCCCAGATCCACGGCCG34570.18976602842263646No Hit
CACCATCACCAGTCTGCAACCTGAAGATTCTGCAGCTTACTACTGTCAAC34450.18910730920335045No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33270.1826299035470383No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA33110.181751611254657No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAACTTTTTAAATTGGTATGAGCAGAAA31900.17510952579352335No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29950.16440533848012617No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG29920.16424065867530466No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAA29680.1629232202367327No Hit
GCTTACTACTGTCAACAGAGTTACACTCCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA29260.16061770296923178No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29150.16001387701821962No Hit
GATTTCACACTCACCATCACCAGTCTGCAACCTGAAGATTCTGCAGCTTACTACTGTCAACAGAGTTACACTCCC29020.15930026453065982No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28880.15853175877482617No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28840.15831218570173083No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT28130.1544147636542888No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27870.15298753867916914No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC27710.15210924638678786No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27440.15062712814339438No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27320.14996840892410843No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26930.14782757146142897No Hit
GTGTGAAATCTGTCCCAGATCCACGGCCGCTGAACCTTGGTGGGACCCCA26910.1477177849248813No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG26710.14661991955940468No Hit
GGCCAAAAGTGTACGGGGGAGTGTAACTCTGTTGACAGTAGTAAGCTGCA26650.14629055994976167No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA25510.14003272736654487No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25120.13789188990386542No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC24500.13448850727088785No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23580.1294383265896953No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23260.1276817420049327No Hit
GGTGATGGTGAGTGTGAAATCTGTCCCAGATCCACGGCCGCTGAACCTTG23250.1276268487366589No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT22170.12169837576308505No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21290.11686776815498785No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21100.11582479605778503No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC20600.11308013264409345No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20260.11121376152278319No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20180.11077461537659253No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20070.11017078942558037No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA19940.10945717693802055No Hit
GATCAGGACCTGAGGGGCTTTCCCTGGTTTCTGCTCATACCAATTTAAAA19880.10912781732837758No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19610.10764569908498411No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19330.10610868757331682No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19270.10577932796367383No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19210.10544996835403084No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT19160.10517550201266168No Hit
CCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC19010.10435210298855421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)