FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821717
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69500.3815082145031308No Hit
GAGTTACACTCCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT56820.3119035503319122No Hit
GTGTAACTCTGTTGACAGTAGTAAGCTGCAGAATCTTCAGGTTGCAGACTGGTGATGGTGAGTGTGAAATCTGTC51750.2840726633170794No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51720.28390798351225793No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG43310.2377427448939654No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39410.216334370267171No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37500.20584975602686914No Hit
CCATAGATCAGGACCTGAGGGGCTTTCCCTGGTTTCTGCTCATACCAATT37330.20491657046621403No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36400.19981149651674768No Hit
CACCATCACCAGTCTGCAACCTGAAGATTCTGCAGCTTACTACTGTCAAC35010.19218133222668504No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34920.19168729281222055No Hit
GATCTATGGTGCATCCAATTTGCAAGGTGGGGTCCCACCAAGGTTCAGCG33760.18531967369245608No Hit
GTAGTAAGCTGCAGAATCTTCAGGTTGCAGACTGGTGATGGTGAGTGTGAAATCTGTCCCAGATCCACGGCCG32960.18092821223054953No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31020.17027891818542615No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA30470.16725978843036543No Hit
GTGTGAAATCTGTCCCAGATCCACGGCCGCTGAACCTTGGTGGGACCCCA29760.16336236638292337No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAACTTTTTAAATTGGTATGAGCAGAAA29690.16297811350500654No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27550.15123095409440654No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAA27520.15106627428958505No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27260.14963904931446542No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26900.14766289165660748No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG26820.14722374551041684No Hit
GATTTCACACTCACCATCACCAGTCTGCAACCTGAAGATTCTGCAGCTTACTACTGTCAACAGAGTTACACTCCC26390.14486333497464204No Hit
GCTTACTACTGTCAACAGAGTTACACTCCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA26370.1447535484380944No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26250.14409482921880842No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25810.1416795254147598No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC25210.1383859293183299No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA25190.13827614278178224No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG25070.13761742356249626No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25040.13745274375767474No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT24950.13695870434321028No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24470.13432382746606636No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23880.13108512463791028No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23700.13009704580898132No Hit
GGCCAAAAGTGTACGGGGGAGTGTAACTCTGTTGACAGTAGTAAGCTGCA23350.1281757814193972No Hit
GGTGATGGTGAGTGTGAAATCTGTCCCAGATCCACGGCCGCTGAACCTTG22990.12619962376153926No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22760.12493707859124112No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21730.11928307195903645No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20630.11324481244891496No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC20560.11286055957099814No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20260.11121376152278319No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19830.10885335098700841No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19800.1086886711821869No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA19420.1066027269877813No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19210.10544996835403084No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC19000.10429720972028038No Hit
GATCAGGACCTGAGGGGCTTTCCCTGGTTTCTGCTCATACCAATTTAAAA18650.10237594533069627No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18540.10177211937968411No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18310.10050957420938599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAC200.00607089854.33128463
GGTATCA19500.039.069241
TGGTATC6800.037.839382
GTGGTAT7100.036.721421
GTATCAA43150.032.95761
TATCAAC48750.028.6823542
AATGGTA10350.028.35318670
ACGGGTC2650.028.0862735
ATCAACG50200.027.7742253
TCAACGC49900.027.7293744
CAACGCA51050.027.1709865
CTAGTAC500.008498793527.0731323
TATGTCG500.008511323527.0649535
AACGCAG51700.026.8293766
AGAGTAC55650.025.46868711
ACGCAGA54650.025.3811327
CGCAGAG55150.025.1510248
AGGGTAT1101.3590488E-624.6227552
GCAGAGT56850.024.51669
TGTTAGA17200.023.56093270
Produced by FastQC (version 0.11.5)