FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439075
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31680.22014141028090958No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG31560.21930754130257282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29920.20791133193197017No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29430.20450636693709498No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAAATTGTTGCTGCCTGCATATGAGCTG28820.20026753296388303No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAAATTGTTGCTGCCTGCATATGAGCTGCAGTAATAAT28170.19575074266455886No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26370.18324270798950715No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26350.183103729826451No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG25230.17532095269530776No Hit
GTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAAATACA23270.16170109271580702No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22040.15315393568785504No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21880.152042110383406No Hit
GATTATTACTGCAGCTCATATGCAGGCAGCAACAATTTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT21520.14954050344839567No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT21510.1494710143668676No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21290.14794225457325016No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20830.1447457568229592No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC20590.14307801886628563No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19460.1352257526536143No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18870.13112589684345846No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18850.13098691868040233No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC18840.13091742959887429No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18820.13077845143581815No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18720.1300835606205375No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCGGTGTGAAGTGCAGCTGGTGGAGT18570.12904122439761653No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT17930.12459392317982036No Hit
CAGTAATACAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAAATA17440.1211889581849452No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17240.11979917655438388No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16980.11799246043465422No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16740.11632472247798063No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16630.11556034258117193No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16330.11347567013532998No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16130.11208588850476868No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15920.11062661779267932No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA15860.11020968330351093No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATCAT15720.10923683616211803No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15490.10763858728697254No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15480.10756909820544447No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15390.10694369647169187No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC15000.10423362229209736No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGATTATGCCATGCACTGGGTCCGGCAAGCTCCAGGGAAGGGC14960.10395566596598509No Hit
GGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAG14680.10200997168319928No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14670.10194048260167121No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14640.10173201535708702No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14630.10166252627555895No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14530.1009676354602783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4600.041.7260741
TGGTATC4750.041.128562
GGTATCA13550.038.44871
GTATCAA32150.036.9935341
GGATGGG14550.035.33078470
TATCAAC35850.032.6963462
ATCAACG37400.031.4110623
TCAACGC37550.031.091274
CAACGCA38150.030.5125455
AACGCAG38600.030.1568286
ACGCAGA40850.028.4958047
CGCAGAG41200.028.4199268
AGAGTAC41450.028.40976111
CAGAGTA41550.028.26096310
GCTTGTA1700.028.2264651
GCAGAGT42400.027.613679
GTGAGCG7400.026.64524570
ATGGCAA2656.21003E-926.57342570
AATACGT1306.852133E-926.33422310
ATACGTA1306.855771E-926.33239411
Produced by FastQC (version 0.11.5)