FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237020
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49990.4041163441173142No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45510.36790027647087353No Hit
GAGTTACAGTACCCTTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGAACTGTGGCTGCACCAT37560.3036329242857836No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33810.2733181355192317No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32790.26507251297472956No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31180.25205736366429No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30880.24963218056296582No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30510.24664112140466604No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27260.22036830447365444No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGCAA26590.2149520622140305No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26390.2133352734798144No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26210.2118801636190199No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC25810.2086465861505877No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25000.2020985917770125No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24770.20023928473266397No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23990.1939338086692212No Hit
ACAGTACCCTTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGA23880.19304457486540233No Hit
CATTTCACTCTTTCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC23290.18827504809946485No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22560.18237376921957607No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22480.18172705372588965No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22260.17994858611825193No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21880.17687668752324134No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGAAAGAGTGAAATGTGTC21570.1743706649852064No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21200.17137960582690662No Hit
GTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21020.16992449596611212No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCGGTGGGTCTGGGACACATTTCA20890.16887358328887164No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC20840.16846938610531761No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAAAGGGTA20800.16814602835847442No Hit
GTCTGGGACACATTTCACTCTTTCCATCAGCAGTCTGCAACCTGAAGATT20600.16652923962425828No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20430.1651549692001746No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT19860.16054712130765872No Hit
GTTCAGTGGCGGTGGGTCTGGGACACATTTCACTCTTTCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA19830.1603046029975263No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19580.1582836170797562No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCTTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGAA19490.15755606214935894No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19020.15375660862395113No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18100.14631938044655707No Hit
GCATAGATCACGAGCTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAATT18070.14607686213642465No Hit
GTAATAAACACCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA17740.14340916072496807No Hit
GAGTGAAATGTGTCCCAGACCCACCGCCACTGAACCTTGACGGGACCCCA17650.14268160579457081No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17350.14025642269324667No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17060.13791207902863334No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16990.13734620297165767No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16890.13653780860454964No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGAAAGAGTGAAATGTGTCCCAGACCCACCGCCA16610.1342743043766471No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCGGTGGGTCTGGGACACATTTCACTCTTT16370.13233415789558778No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16340.13209163958545536No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15850.1281305071866259No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15840.12804966774991514No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT15240.12319930154726681No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA15120.12222922830673717No Hit
GTCCCAGACCCACCGCCACTGAACCTTGACGGGACCCCACTTTGCAAACT14940.12077411844594267No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14770.11939984802185899No Hit
CCACTGAACCTTGACGGGACCCCACTTTGCAAACTGGATGCAGCATAGAT14750.11923816914843738No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14640.11834893534461852No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14610.1181064170344861No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14580.11786389872435368No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14560.11770221985093207No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14520.11737886210408886No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14470.11697466492053482No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAG14460.11689382548382404No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14350.11600459168000517No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14330.11584291280658356No Hit
GTTACAGTACCCTTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAC14040.11349856914197021No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCTTTGGACGTTCGGCCAAGGGA13890.11228597759130815No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13250.10711225364181663No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13250.10711225364181663No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13230.10695057476839502No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13220.10686973533168422No Hit
GTCCAAAGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTC13080.10573798321773295No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12910.10436371279364925No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12830.10371699729996282No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12640.10218104800245752No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12580.10169601138219268No Hit
GTGTTTGGTGGGTCCTTCAGTGGTTACTTCTGGACCTGGATCCGCCAGCC12510.10113013532521706No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12430.1004834198315306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)