FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237020
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48610.3929605018512231No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42210.3412232623563079No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35580.2876267158170442No Hit
GAGTTACAGTACCCTTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGAACTGTGGCTGCACCAT35510.2870608397600686No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34040.2751774425635802No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30910.24987469887309827No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG30390.2456710481641364No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28590.23111994955619147No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26140.21131428756204426No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCACCTATTTAAATTGGTATCAGCAGCAA24310.19652067064396694No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24230.1958739551502805No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC24010.1940954875426428No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23180.187385814295646No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22920.18528398894116507No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21920.17720004527008454No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21530.17404730723836317No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21470.17356227061809834No Hit
ACAGTACCCTTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGA21330.17243051850414706No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21070.17032869314966614No Hit
GTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21050.17016701427624453No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGAAAGAGTGAAATGTGTC20690.16725679455465553No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20610.1666100790609691No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20600.16652923962425828No Hit
CATTTCACTCTTTCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC20470.16547832694701783No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20260.16378069877609092No Hit
GTCTGGGACACATTTCACTCTTTCCATCAGCAGTCTGCAACCTGAAGATT20190.1632148227191153No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20140.16281062553556128No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAC19730.15949620863041827No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19710.15933452975699666No Hit
GTTCAGTGGCGGTGGGTCTGGGACACATTTCACTCTTTCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA19310.15610095228856444No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19140.15472668186448077No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19100.15440332411763755No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCGGTGGGTCTGGGACACATTTCA18960.1532715720036863No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAAAGGGTA18190.1470469353769543No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18070.14607686213642465No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCTTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAACCGAA17970.14526846776931657No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17910.14478343114905173No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17720.14324748185154645No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16780.13564857480073078No Hit
GAGTGAAATGTGTCCCAGACCCACCGCCACTGAACCTTGACGGGACCCCA16660.13467850156020114No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16090.13007065366768525No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGAAAGAGTGAAATGTGTCCCAGACCCACCGCCA15920.12869638324360155No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15600.1261095212688558No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCGGTGGGTCTGGGACACATTTCACT15590.126028681832145No Hit
GTAATAAACACCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA15540.12562448464859097No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15540.12562448464859097No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCGGTGGGTCTGGGACACATTTCACTCTTT15440.12481609028148291No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15100.12206754943331555No Hit
GCATAGATCACGAGCTTAGGGGCTTTCCCTGGTTGCTGCTGATACCAATT15080.12190587055989394No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14710.11891481140159416No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14490.11713634379395645No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14460.11689382548382404No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA14360.11608543111671597No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14040.11349856914197021No Hit
GTCCCAGACCCACCGCCACTGAACCTTGACGGGACCCCACTTTGCAAACT13420.10848652406590031No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13410.1084056846291895No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13410.1084056846291895No Hit
CCACTGAACCTTGACGGGACCCCACTTTGCAAACTGGATGCAGCATAGAT13060.10557630434431131No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13050.10549546490760053No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13050.10549546490760053No Hit
GTGTTTGGTGGGTCCTTCAGTGGTTACTTCTGGACCTGGATCCGCCAGCC12740.10298944236956557No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12710.10274692405943316No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12630.1021002085657467No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12610.1019385296923251No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12580.10169601138219268No Hit
GTTACAGTACCCTTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAC12530.10129181419863867No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAG12450.10064509870495222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)