FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759732
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCTCGGACGTTCGGCCAAGGGA67300.3824445995185631No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52010.2955563688107053No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA46620.264926704748223No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42980.24424173680992334No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA42230.23997972418527366No Hit
GTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTAATTTGCAGATACA34730.19735959793877705No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34020.19332489265410868No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31560.1793454912452578No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA31210.1773565520204213No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30920.17570857380555677No Hit
GTATGGTAGCTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT30630.17406059559069223No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29350.16678676071129014No Hit
CTCCTGCAGGGCCAATCAGAGTGTTCGCAGCAGGTACTTAGCCTGGTACC28480.1618428260666965No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT27780.15786494761702352No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA27720.15752398660705152No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27360.1554782205472197No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26900.15286418613743455No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26860.15263687879745325No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT26350.14973871021269147No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26240.14911361502774287No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25560.1452493902480605No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25500.14490842923808853No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25160.14297631684824735No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24970.1418966069833361No Hit
TGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGT24880.14138516546837815No Hit
GTATTACTGTGCAAAAGATGCGTCGGATATAGCAGTGACTGGTTCGTACTTTGACTACTGGGGCCAGGGAACC24870.14132833863338282No Hit
GTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCA24530.13939622624354164No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG24510.13928257257355098No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24320.13820286270863974No Hit
GTCTCCAGGCACTCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAATCAGAGTGTTCGCAG24270.13791872853366308No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT24250.13780507486367244No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24130.13712315284372847No Hit
CCATAGATGAGGAGCCTGGGACCCTGGCCAGGTTGTTGCTGGTACCAGGC23960.1361570966488079No Hit
CTCCAGGCACTCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC23900.13581613563883593No Hit
CAGTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTAATTTGCAGATA23670.13450911843394336No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23530.13371354274400873No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAATCAGA23490.13348623540402743No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23230.1320087376941489No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23100.1312699888392096No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23000.13070172048925632No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAG22190.12609874685463468No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22110.12564413217467205No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC21820.12399615395980751No Hit
TTGTATTACTGTGCAAAAGATGCGTCGGATATAGCAGTGACTGGTTCGTACTTTGACTACTGGGGCCAGGGAACC21790.12382567345482152No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21490.12212086840496167No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21190.1204160633551018No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC21010.11939318032518588No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20970.11916587298520458No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT19820.11263078696074176No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTACAACTGGTGGAGT19080.10842560117108743No Hit
GTACTTAGCCTGGTACCAGCAACAACCTGGCCAGGGTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCA18990.10791415965612945No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC18910.10745954497616683No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18900.1074027181411715No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC18850.10711858396619486No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18470.10495916423637235No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA18430.10473185689639104No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTGATGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGT18340.10422041538143308No Hit
GCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTC18310.10404993487644709No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18290.10393628120645643No Hit
GGTCTGGAGTGGGTCTCTCTTATTAGTTGGGATGGTGGTAGCACATCTTA18220.10353849336148914No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18170.10325435918651249No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18110.10291339817654051No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT18040.10251561033157322No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17990.10223147615659657No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17970.10211782248660592No Hit
CTCTTATTAGTTGGGATGGTGGTAGCACATCTTATGCAGACTCTGTGAAG17840.10137907363166664No Hit
GCTATATCCGACGCATCTTTTGCACAGTAATACAAGGCGGTGTCCTCAGT17670.10041301743674606No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA17660.10035619060175072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)