FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759732
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCTCGGACGTTCGGCCAAGGGA58450.33215285054769705No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49220.27970168184700855No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA42370.24077529987520827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38890.22099956129683382No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA37210.21145265301761856No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35060.19923488349362287No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32890.18690346029963653No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32180.1828687550149682No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA29580.16809377791618269No Hit
GTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTAATTTGCAGATACA29030.1649683019914396No Hit
GTATGGTAGCTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT28540.1621837870766685No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28420.16150186505672454No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28100.15968340633687403No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT27000.15343245448738785No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26350.14973871021269147No Hit
CTCCTGCAGGGCCAATCAGAGTGTTCGCAGCAGGTACTTAGCCTGGTACC24320.13820286270863974No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23960.1361570966488079No Hit
GTATTACTGTGCAAAAGATGCGTCGGATATAGCAGTGACTGGTTCGTACTTTGACTACTGGGGCCAGGGAACC23860.1355888282988546No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23740.13490690627891064No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT23670.13450911843394336No Hit
CTCCAGGCACTCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC23410.1330316207240648No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAATCAGA23060.1310426814992283No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23010.13075854732425166No Hit
GTCTCCAGGCACTCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAATCAGAGTGTTCGCAG22930.13030393264428902No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22780.1294515301193591No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22470.12768989823450388No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21680.12320057826987292No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21580.12263230991991962No Hit
CCATAGATGAGGAGCCTGGGACCCTGGCCAGGTTGTTGCTGGTACCAGGC21490.12212086840496167No Hit
TGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGTCTGGAGT21440.12183673422998503No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21390.12155260005500838No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21310.12109798537504575No Hit
GTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCA21130.12007510234512983No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21060.11967731450016253No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21000.11933635349019056No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20960.11910904615020923No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC20730.11780202894531669No Hit
TTGTATTACTGTGCAAAAGATGCGTCGGATATAGCAGTGACTGGTTCGTACTTTGACTACTGGGGCCAGGGAACC20460.11626770440044279No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20350.11564260921549417No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20170.11461972618557825No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAG20040.11388097733063898No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC19990.11359684315566233No Hit
CAGTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTAATTTGCAGATA19880.11297174797071372No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19640.11160790393082583No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTACAACTGGTGGAGT19290.10961896470598934No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTGATGATTATACCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGT19240.10933483053101269No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19190.10905069635603604No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19130.10870973534606405No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19050.10825512066610142No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18900.1074027181411715No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18870.1072322376361855No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18370.10439089588641906No Hit
CTCTTATTAGTTGGGATGGTGGTAGCACATCTTATGCAGACTCTGTGAAG18100.10285657134154519No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18010.10234512982658724No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17970.10211782248660592No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA17920.10183368831162927No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17910.10177686147663395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTA200.00764120651.2609515
TGGTATC3850.049.7245522
GTGGTAT4300.043.744471
GGTATCA11950.043.2153431
GTATCAA35250.041.62241
TATCAAC40950.035.396112
ATCAACG41800.034.4970633
GGCGTAA400.002709101234.2031425
TCAACGC42400.034.0749554
CAACGCA43100.033.4394345
AACGCAG43350.033.3253756
ACGCAGA45250.032.0015537
CGCAGAG45950.031.5883678
TACCTAT900.004349768631.04962370
AGAGTAC46650.030.89298811
GCAGAGT48150.030.1450799
ACAGGTA3150.029.3144781
GTTATTA1000.00727933327.9446670
CAGAGTA54900.026.31429910
AACTGTG33850.025.38547770
Produced by FastQC (version 0.11.5)