FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390901
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGGCTGGAGCCCACTGATGGCCAGGGAGG72060.5180814450489287No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAAAGGTGCTGTGTTCGG61770.444100622546105No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAGCACCTTTCAGGCTGTCATCCCATG53610.38543361461383663No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC53120.38191071830417833No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC37690.27097543247146993No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTATTCATTAGCATATACA31850.2289882601277877No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT31630.2274065515805942No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTC31110.22366796774177314No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT28920.20792277811289228No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT28870.20756329889762104No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27390.19692271412559198No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG27290.19620375569504947No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26170.1881514212729734No Hit
GTATATGCTAATGAATAGCCTGAGAGCCGAGGACACGGCCGTATATTACT25370.18239975382863338No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG25370.18239975382863338No Hit
GTATATTACTGTGCGAAAGATTTTTTCTTCTTTCCAGTGGGGGGTACTAT24780.1781578990884326No Hit
GTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAG23720.17053693972468204No Hit
GGATATTTATTGAGAAGGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGCAGCTCCAGG23680.17024935635246505No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23400.16823627274694605No Hit
GATTATTACTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGG23160.16651077251364405No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGA22940.16492906396645052No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22800.16392252216369102No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC22660.16291598036093152No Hit
GGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGC22590.16241270945955175No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA21740.15630156279994048No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTGAC20390.14659562398761666No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT20070.14429495700988065No Hit
GTGTTACTTCCGATGTTGGACCTGCTTCCAGAACAAGAGATGGTCACCCT20030.14400737363766364No Hit
AGCATATACAGCGTGTTCTTGGGATTGTCCCTGGAGATGGTGAACCGGCCCTTCACGGAGTCTGCGTAGTATGTG19780.14220997756130738No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT19730.14185049834603614No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC19720.14177860250298188No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA19360.13919035215302886No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19090.13724916439056412No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGC19080.13717726854750986No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19080.13717726854750986No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC19050.1369615810183471No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19050.1369615810183471No Hit
CTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAGACTCCTCATC18930.13609883090169608No Hit
GCATGGGATGACAGCCTGAAAGGTGCTGTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTCAGCCCAAGG18810.1352360807850451No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG18690.13437333066839408No Hit
CCCCTCCCCTGGGAACCTGCAGCTCCAGGCCCCTGTGGGTGGGGTGAGGG18610.13379816392396007No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGTGACTTCTACC17550.12617720456020953No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTT17410.12517066275745No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGTGTTACTT17360.12481118354217878No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC17280.12423601679774478No Hit
GAAGTAACACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAGA16920.12164776644779174No Hit
GGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGG16900.12150397476168326No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC16350.1175497033936995No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTGGGTG16050.11539282810207198No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG16000.11503334888680072No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTACGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15830.11381111955487846No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGG15800.11359543202571569No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15570.11194182763546795No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15510.11151045257714244No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC15460.11115097336187119No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGT15380.11057580661743718No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC15270.10978495234384043No Hit
CTCCAGGCCCCTGTGGGTGGGGTGAGGGTCGGGGGCCTAAGAGCATTCTG15090.10849082716886391No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC14910.10719670199388742No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGCCTGAGGATG14860.10683722277861617No Hit
GGCCATCAGTGGGCTCCAGCCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAAAGGTG14800.10640584772029066No Hit
CTATAGGGACGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG14760.10611826434807364No Hit
GCCATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14550.10460845164393441No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14500.10424897242866316No Hit
GTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC14420.10367380568422914No Hit
CCACCAAACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTAC14350.10317053478284938No Hit
GGCAGAGGGTGACCATCTCTTGTTCTGGAAGCAGGTCCAACATCGGAAGTAACACTGTAAACTGGTACCAGCAG14210.10216399298008988No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT13960.10036659690373362No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTGACCATCTCTTGTTCTGG13960.10036659690373362No Hit
GCCCCCAGACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT13910.10000711768846236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)