FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390901
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAAAGGTGCTGTGTTCGG57400.4126821391313975No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC52680.37874730120979133No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48400.34797588038257216No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAGCACCTTTCAGGCTGTCATCCCATG44170.3175639387706242No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC39310.2826225590462585No Hit
GTAATAATCAGCCTCATCCTCAGGCTGGAGCCCACTGATGGCCAGGGAGG35690.25659626386061984No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT31270.22481830123064114No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTATTCATTAGCATATACA30280.21770061276827035No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT29310.21072671599200807No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT27040.1944063596186932No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG26690.19189000511179444No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTC25510.18340629563139288No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG25460.18304681641612164No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24280.17456310693572008No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTGAC24040.17283760670241807No Hit
GTATATGCTAATGAATAGCCTGAGAGCCGAGGACACGGCCGTATATTACT23260.16722973094418656No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC23010.16543233486783027No Hit
GGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGC21970.15795516719018823No Hit
GTATATTACTGTGCGAAAGATTTTTTCTTCTTTCCAGTGGGGGGTACTAT21800.15673293785826597No Hit
GTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAG21310.1532100415486077No Hit
GGATATTTATTGAGAAGGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGCAGCTCCAGG21140.15198781221668545No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21030.15119695794308868No Hit
CTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAGACTCCTCATC20860.14997472861116642No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20850.14990283276811217No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT20390.14659562398761666No Hit
GTGTTACTTCCGATGTTGGACCTGCTTCCAGAACAAGAGATGGTCACCCT20160.1449420195973689No Hit
GATTATTACTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGG20010.14386358195155516No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGA19900.1430727276779584No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA19720.14177860250298188No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG19670.1414191232877106No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19480.14005310226967987No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC19350.1391184563099746No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA19010.13667399764613009No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGTGTTACTT18620.13387005976701433No Hit
GAAGTAACACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAGA17930.12890924659627104No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGC17660.1269680588338063No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC17540.12610530871715528No Hit
AGCATATACAGCGTGTTCTTGGGATTGTCCCTGGAGATGGTGAACCGGCCCTTCACGGAGTCTGCGTAGTATGTG17510.12588962118799252No Hit
GCATGGGATGACAGCCTGAAAGGTGCTGTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTCAGCCCAAGG17440.1253863502866128No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17380.12495497522828727No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17300.12437980848385326No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTT17120.12308568330887677No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGG16780.12064122464503224No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGTGACTTCTACC16680.11992226621448974No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC16480.11848434935340472No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16390.11783728676591648No Hit
GGGTGAGGGTCGGGGGCCTAAGAGCATTCTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16130.11596799484650598No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC15940.11460197382847521No Hit
GGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGACGGGG15870.11409870292709545No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15370.11050391077438294No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGCCTGAGGATG15340.1102882232452202No Hit
GGCAGAGGGTGACCATCTCTTGTTCTGGAAGCAGGTCCAACATCGGAAGTAACACTGTAAACTGGTACCAGCAG15180.10913788975635218No Hit
CTCTTGTTCTGGAAGCAGGTCCAACATCGGAAGTAACACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCC15150.10892220222718943No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15090.10849082716886391No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15050.10820324379664692No Hit
CCCCTCCCCTGGGAACCTGCAGCTCCAGGCCCCTGTGGGTGGGGTGAGGG15040.10813134795359268No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT14890.10705291030777891No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA14780.10626205603418215No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC14390.10345811815506639No Hit
CTATAGGGACGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG14330.1030267430967409No Hit
GTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC14280.10266726388146964No Hit
GGCCATCAGTGGGCTCCAGCCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAAAGGTG13940.10022280521762511No Hit
GGGTGACCATCTCTTGTTCTGGAAGCAGGTCCAACATCGGAAGTAACACT13910.10000711768846236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4300.048.3787881
TGGTATC4250.047.3260732
GGTATCA15800.044.0318221
TCGTCCG350.00142882238.96373420
GTATCAA38750.038.3714681
GGTCTAG451.07229804E-437.892311
GACAGGT2850.033.504781
TATCAAC44750.032.5291142
AATGGTA2009.662472E-932.36647870
ATCAACG46700.031.4492513
TCAACGC46800.031.0852154
AACGCAG47500.030.7694646
CAACGCA47400.030.6906285
GCTCCTA604.1290917E-430.11983566
TAGTAGC604.4380705E-429.75106863
CTAATCG605.080482E-429.07204249
CGCAGAG51700.028.4675088
CTTTCCA13450.028.34239470
GCCAGAG21750.028.10881870
AGAGTAC53700.027.91480611
Produced by FastQC (version 0.11.5)