FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285985
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50550.39308390066758164No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49210.3826638724401918No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38220.2972040886946582No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36270.2820406147816654No Hit
GAGTTCACTCTCACCATCGACAACCTGCAGCCTGATGATTCTGCAACTTA34360.2671881864874007No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33240.2584789091630151No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32690.25420203190550433No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32670.25404650909614035No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32560.25319113364463813No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32470.25249128100250007No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29980.2331286912366785No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28610.22247537879524254No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27370.21283296461467283No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27330.21252191899594472No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26960.20964474702271022No Hit
ACTTATTACTGCCTACACTATCACAGCTATTCTCTCATATTCACTTTCGGCCCTGGGACCAAGGTGGATTTCATT25220.19611426260803974No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGGGTCACCCTCACTTGCCG24360.18942678180538652No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24070.18717170106960812No Hit
CCCCTAGACTCCTGATCTATAAGGCGTCTCGTTTAGAAAGTGGGGTCCCA23610.18359467645423547No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23290.18110631150441103No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGAATGAAATCCACCTTGGTCC22830.17752928688903835No Hit
GTAATAAGTTGCAGAATCATCAGGCTGCAGGTTGTCGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCG22510.17504092193921392No Hit
GAGTATTAATAACTGGTTGGCCTGGTATCAGCAGAGACCGGGGAAGGCCCCTAGACTCCTGATCTATAAGGCGT22200.17263031839407147No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGGCAAATGTGACAT22140.17216374996597938No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21480.16703149725696645No Hit
GTGCAGCCACAGTTCGAATGAAATCCACCTTGGTCCCAGGGCCGAAAGTG21360.16609836040078227No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21110.16415432528373192No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20970.16306566561818372No Hit
GTCTAGGGGCCTTCCCCGGTCTCTGCTGATACCAGGCCAACCAGTTATTA20610.1602662550496312No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20550.15979968662153912No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20370.15839998133726288No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20310.1579334129091708No Hit
GTCTGCATCTGTAGGAGACAGGGTCACCCTCACTTGCCGGGCCAGTCAGA20260.15754460588576072No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19070.14829099872860102No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18730.14564711096941255No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18320.14245889337744996No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18080.14059261966508163No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17960.13965948280889748No Hit
GTAGGAGACAGGGTCACCCTCACTTGCCGGGCCAGTCAGAGTATTAATAACTGGTTGGCCTGGTATCAGCAGAGA17840.13872634595271327No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17730.13787097050121114No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17690.13755992488248306No Hit
GGTATCAGCAGAGACCGGGGAAGGCCCCTAGACTCCTGATCTATAAGGCGTCTCGTTTAGAAAGTGGGGTCCCAG17250.13413842307647444No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17190.13367185464838235No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGGCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC17100.13297200200624423No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17000.13219438795942412No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16900.13141677391260398No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16850.13102796688919388No Hit
GGGTCACCCTCACTTGCCGGGCCAGTCAGAGTATTAATAACTGGTTGGCCTGGTATCAGCAGAGACCGGGGAAGG16550.12869512474873346No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16450.12791751070191332No Hit
CTCCTGATCTATAAGGCGTCTCGTTTAGAAAGTGGGGTCCCAGCCAGGTT16070.12496257732399678No Hit
ATTAATAACTGGTTGGCCTGGTATCAGCAGAGACCGGGGAAGGCCCCTAG16000.12441824749122268No Hit
GTTATTAATACTCTGACTGGCCCGGCAAGTGAGGGTGACCCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA15890.12356287203972054No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTGGCTGGGACCCCA15810.12294078080226441No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15740.12239645096949031No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15660.1217743597320342No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15640.12161883692267017No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15480.12037465444775794No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15300.1189749491634817No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14800.11508687892938098No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14490.11267627538423854No Hit
GTGATAGTGTAGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGTTGT14170.11018791043441407No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14040.1091770121735479No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14030.1090992507688659No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14000.10886596655481984No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13970.1086326823407738No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13730.10676640862840546No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13580.10559998755817525No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13570.10552222615349324No Hit
CTGCTGATACCAGGCCAACCAGTTATTAATACTCTGACTGGCCCGGCAAG13390.10412252086921699No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13360.10388923665517094No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGGGTCACCCTCACTTGCCGGGCCAGTCAGAGTATTAATAA13360.10388923665517094No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13280.10326714541771484No Hit
GTATTAATAACTGGTTGGCCTGGTATCAGCAGAGACCGGGGAAGGCCCCT13240.10295609979898677No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13190.1025672927755767No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13160.10233400856153065No Hit
CACTATCACAGCTATTCTCTCATATTCACTTTCGGCCCTGGGACCAAGGTGGATTTCATTCGAACTGTGGCTGCA13070.10163415591939254No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12980.1009343032772544No Hit
CCTTATAGATCAGGAGTCTAGGGGCCTTCCCCGGTCTCTGCTGATACCAGGCCAACCAGTTATTAATACTCTGA12930.10054549625384433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAA200.00646803253.46696558
CGCGTAT200.0074273351.62953639
CCGCGTT400.007583249451.35598470
GTATCAA36000.039.114951
TCGTATG2900.036.85890240
CGTATGC3300.036.6401841
AGACGTA4500.036.236825
CGTAGAG4450.035.9239128
GAGACGT4550.035.82603524
ATCTCGT2850.034.91333837
ACGTAGA4600.034.73081227
GTCGTAT501.8871945E-434.4196939
GACGTAG4650.034.3439826
AATCGCG501.9380712E-434.26421736
TCTCCGA4900.033.91916311
GACTGAA4450.033.85263470
ATGCCGT3300.033.64749544
TATCAAC42350.033.089772
ATCAACG42400.033.050753
TCAACGC42750.032.8569764
Produced by FastQC (version 0.11.5)