FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285985
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46660.3628347142462781No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42540.3307970155172883No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39600.30793516254077613No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37260.2897389938451848No Hit
GAGTTCACTCTCACCATCGACAACCTGCAGCCTGATGATTCTGCAACTTA35360.27496432695560213No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33670.26182264956434176No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31260.24308215103597633No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29030.22574135779188714No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28150.21889835417986991No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27830.21640998923004545No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27380.2129107260193548No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26860.20886713297589005No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26570.20661205224011164No Hit
ACTTATTACTGCCTACACTATCACAGCTATTCTCTCATATTCACTTTCGGCCCTGGGACCAAGGTGGATTTCATT25400.197513967892316No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25250.1963475468220858No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTGGCTGGGACCCCA23880.18569423438064986No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23710.1843722905010556No Hit
GTAATAAGTTGCAGAATCATCAGGCTGCAGGTTGTCGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCG23280.181028550099729No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22920.1782291395311765No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22690.17644062722349016No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22310.1734856938455736No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21650.1683534411365607No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21350.16602059899610025No Hit
GTGCAGCCACAGTTCGAATGAAATCCACCTTGGTCCCAGGGCCGAAAGTG21090.16399880247436788No Hit
CCCCTAGACTCCTGATCTATAAGGCGTCTCGTTTAGAAAGTGGGGTCCCA20550.15979968662153912No Hit
GAGTATTAATAACTGGTTGGCCTGGTATCAGCAGAGACCGGGGAAGGCCCCTAGACTCCTGATCTATAAGGCGT20470.15917759538408302No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20180.1569225146483046No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19760.15365653565166001No Hit
GTCTAGGGGCCTTCCCCGGTCTCTGCTGATACCAGGCCAACCAGTTATTA19590.15233459177206576No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGGGTCACCCTCACTTGCCG19500.15163473912992764No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGAATGAAATCCACCTTGGTCC19400.1508571250831075No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19340.1503905566550154No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19260.1497684654175593No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGGCAAATGTGACAT18520.14401412147109025No Hit
GTCTGCATCTGTAGGAGACAGGGTCACCCTCACTTGCCGGGCCAGTCAGA18350.14269217759149602No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17940.13950395999953344No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17130.1332052862202903No Hit
GTCCCAGATCCACTGCCGCTGAACCTGGCTGGGACCCCACTTTCTAAACG17080.13281647919688022No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16750.13025035284237374No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16720.1300170686283277No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16480.12815079491595938No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16310.12682885103636513No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16280.12659556682231907No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16110.12527362294272484No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15940.12395167906313061No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15890.12356287203972054No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15790.12278525799290038No Hit
GTTATTAATACTCTGACTGGCCCGGCAAGTGAGGGTGACCCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA15610.12138555270862413No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15480.12037465444775794No Hit
GTAGGAGACAGGGTCACCCTCACTTGCCGGGCCAGTCAGAGTATTAATAACTGGTTGGCCTGGTATCAGCAGAGA15390.11967480180561982No Hit
GGGTCACCCTCACTTGCCGGGCCAGTCAGAGTATTAATAACTGGTTGGCCTGGTATCAGCAGAGACCGGGGAAGG15080.11726419826047736No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15050.11703091404643134No Hit
CTCCTGATCTATAAGGCGTCTCGTTTAGAAAGTGGGGTCCCAGCCAGGTT14860.11555344735747305No Hit
ATTAATAACTGGTTGGCCTGGTATCAGCAGAGACCGGGGAAGGCCCCTAG14680.11415374207319681No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14630.11376493504978674No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14410.11205418414678243No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14070.10941029638759393No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14010.10894372795950186No Hit
GGTATCAGCAGAGACCGGGGAAGGCCCCTAGACTCCTGATCTATAAGGCGTCTCGTTTAGAAAGTGGGGTCCCAG13990.10878820515013783No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGGCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC13940.10839939812672776No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13920.10824387531736374No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGCCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG13660.10622207879563136No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13620.10591103317690331No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13290.10334490682239685No Hit
GTGATAGTGTAGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGTTGT13280.10326714541771484No Hit
CTGCTGATACCAGGCCAACCAGTTATTAATACTCTGACTGGCCCGGCAAG13260.10311162260835079No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13120.10202296294280261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)