FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589671
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC60230.3788834293385235No Hit
GATTATTACTGCTCCTCATATACAGGGAGTGTCACTTGGGTGGCATTCGG59820.3763042793131409No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT54020.33981874236870396No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51940.3267342739472507No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCAC51920.3266084617508906No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT44510.2799950429994634No Hit
GTATATGAGGAGCAGTAATAATCAGCCTCGTCTTCAGCCTGGAGTCCAGA43510.27370443318145704No Hit
GTAATAATCAGCCTCGTCTTCAGCCTGGAGTCCAGAGATTGTCAGGGAGG42410.26678476238165005No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC38510.24225138409142521No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACCCAAGTGACA38360.24130779261872423No Hit
CTATTATGTTGTCTCCTGGTATCAACAATACGCAGGCAAACCCCCCAAACTCATTATTTATGAGGTCAGTAAGCG37700.23715599013884006No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37250.23432521572073717No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT36080.22696520223366973No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC35190.22136655949564404No Hit
ACATAATAGCTCCCAATATCATTACTGGTTCCATAGCAGGGGATGGTGAT34680.2181583484884608No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG34530.21721475701575987No Hit
GTCGATCACCATCCCCTGCTATGGAACCAGTAATGATATTGGGAGCTATTATGTTGTCTCCTGGTATCAACAATA33530.21092414719775351No Hit
CCTCATATACAGGGAGTGTCACTTGGGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG32310.20324960321978572No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCATGCAGCTCTAGTCTCC31940.20092207758712338No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31420.19765096048176006No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30510.19192650554737425No Hit
TCTCTGGACTCCAGGCTGAAGACGAGGCTGATTATTACTGCTCCTCATAT30270.19041675919105272No Hit
CATATACAGGGAGTGTCACTTGGGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG30240.19022804089651255No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29850.18777470306749006No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29190.18362290058760586No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28860.18154699934766377No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG28390.17859041273320075No Hit
GCTCTAGTCTCCCGTGGGGGGGGGTGAGGGTTGAGAACCTATGAACATTC28080.1766403236896188No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27510.17305467609335518No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT27480.17286595779881497No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG26990.16978355898799186No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCCCCTG26810.1686512492207507No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26480.1665753479808086No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC26440.16632372358808836No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCATGCAG26000.16355585526816555No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCC24430.15367959785389554No Hit
CTCCTGGACAGTCGATCACCATCCCCTGCTATGGAACCAGTAATGATATT24380.15336506736299524No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24340.153113442970275No Hit
ATTATGTTGTCTCCTGGTATCAACAATACGCAGGCAAACCCCCCAAACTCATTATTTATGAGGTCAGTAAGCGCC24000.15097463563215283No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23930.15053429294489237No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCATGCAG23790.14965360757037147No Hit
TATACAGGGAGTGTCACTTGGGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC23430.14738898803588918No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22050.1387079464870404No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC21140.1329834915526546No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20150.12675578783282831No Hit
GTCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG19980.1256863841637672No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19720.12405082561108556No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19610.12335885853110487No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACCCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19550.12298142194202448No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19540.12291851584384443No Hit
CCTCAGGGATTTCTAAGCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGACTCCAGG19150.12046517801482194No Hit
CCCCTGGGATCATGCAGCTCTAGTCTCCCGTGGGGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18950.11920705605122067No Hit
CCCTGTATATGAGGAGCAGTAATAATCAGCCTCGTCTTCAGCCTGGAGTC18940.11914414995304058No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18790.11820055848033963No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACCCAAGTGA18270.11492944137497633No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC18000.11323097672411463No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17610.11077763889509214No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17480.10995985961875131No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17470.10989695352057124No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACCCAAGTGACAC17400.10945661083331078No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16900.1063113059243076No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16900.1063113059243076No Hit
CTGTATATGAGGAGCAGTAATAATCAGCCTCGTCTTCAGCCTGGAGTCCAGAGATTGTCAGGGAGGCCGTGTTGC16730.10524190225524653No Hit
CTGCTGCTCCTCACCCTCCTCACCCAGGACACAGGGTCCTGGGCCCAGTC16640.10467574737162595No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC16490.103732155898925No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACCCAGGACACAGGGTCCTGGGC16400.10316600101530442No Hit
GGTATCAACAATACGCAGGCAAACCCCCCAAACTCATTATTTATGAGGTC16400.10316600101530442No Hit
GTAAGCGCCCCTCAGGGATTTCTAAGCGCTTCTCTGGCTCCAAGTCTGGC16250.10222240954260346No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16170.10171916075716297No Hit
GCTATGGAACCAGTAATGATATTGGGAGCTATTATGTTGTCTCCTGGTAT16130.10146753636444271No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15950.10033522659720155No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15930.10020941440084144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)