FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589671
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC58020.36498118164072946No Hit
GATTATTACTGCTCCTCATATACAGGGAGTGTCACTTGGGTGGCATTCGG55940.3518967132192762No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53550.336862155754241No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49220.3096238152422734No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCAC45690.2874179625847109No Hit
GTATATGAGGAGCAGTAATAATCAGCCTCGTCTTCAGCCTGGAGTCCAGA40610.2554616647092386No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT38120.23979804626240273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37960.2387915486915217No Hit
GTAATAATCAGCCTCGTCTTCAGCCTGGAGTCCAGAGATTGTCAGGGAGG36460.22935563396451214No Hit
CTATTATGTTGTCTCCTGGTATCAACAATACGCAGGCAAACCCCCCAAACTCATTATTTATGAGGTCAGTAAGCG36120.22721682662639No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT35950.2261474229573289No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35360.22243596316470515No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG35360.22243596316470515No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC34850.2192277521575219No Hit
GTCGATCACCATCCCCTGCTATGGAACCAGTAATGATATTGGGAGCTATTATGTTGTCTCCTGGTATCAACAATA34360.2161453533466988No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC32700.2057029410488082No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30530.1920523177437344No Hit
ACATAATAGCTCCCAATATCATTACTGGTTCCATAGCAGGGGATGGTGAT30350.19092000797649325No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC29290.1842519615694065No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCCCCTG29280.18418905547122644No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCATGCAGCTCTAGTCTCC29140.18330837009670553No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCATGCAG29090.1829938396058052No Hit
CCTCATATACAGGGAGTGTCACTTGGGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG28720.18066631397314287No Hit
TCTCTGGACTCCAGGCTGAAGACGAGGCTGATTATTACTGCTCCTCATAT28710.18060340787496282No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACCCAAGTGACA28560.17965981640226186No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27660.17399826756605613No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC26940.16946902849709153No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26530.16688987847170894No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25750.16198320281366396No Hit
CATATACAGGGAGTGTCACTTGGGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG24980.15713943325379903No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24870.15644746617381836No Hit
GCTCTAGTCTCCCGTGGGGGGGGGTGAGGGTTGAGAACCTATGAACATTC24710.15544096860293732No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG24640.1550006259156769No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24270.15267310028301453No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCATGCAG24190.15216985149757403No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23970.15078591733761262No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC22910.1441178709305259No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22870.14386624653780564No Hit
ATTATGTTGTCTCCTGGTATCAACAATACGCAGGCAAACCCCCCAAACTCATTATTTATGAGGTCAGTAAGCGCC22570.14197906359240373No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22460.14128709651242302No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22120.13914828917430086No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCC21850.13744982452343912No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20450.12864297077823023No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20260.127447754912809No Hit
CTCCTGGACAGTCGATCACCATCCCCTGCTATGGAACCAGTAATGATATT20240.1273219427164489No Hit
TATACAGGGAGTGTCACTTGGGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC20080.12631544514556786No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACCCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20080.12631544514556786No Hit
CTGCTGCTCCTCACCCTCCTCACCCAGGACACAGGGTCCTGGGCCCAGTC19990.12574929026194728No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19610.12335885853110487No Hit
GTCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG19350.12172329997842321No Hit
CCCTGTATATGAGGAGCAGTAATAATCAGCCTCGTCTTCAGCCTGGAGTC18810.11832637067669977No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC18680.11750859140035894No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACCCAAGTGA18570.11681662432037825No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18370.11555850235677696No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18080.11373422550955511No Hit
CCCCTGGGATCATGCAGCTCTAGTCTCCCGTGGGGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17520.11021148401147156No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17400.10945661083331078No Hit
CCTCAGGGATTTCTAAGCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGACTCCAGG16800.10568224494250697No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16660.10480155956798606No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16310.10259984613168384No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16230.10209659734624335No Hit
CTGTATATGAGGAGCAGTAATAATCAGCCTCGTCTTCAGCCTGGAGTCCAGAGATTGTCAGGGAGGCCGTGTTGC16080.1011530058735424No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACCCAGGACACAGGGTCCTGGGC16060.10102719367718226No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACCCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT15950.10033522659720155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4700.044.957311
GCTTACC400.002757138334.080541
ATCAACG47000.031.5247573
GAGTTAA652.524562E-531.4589611
TCAACGC47500.031.2597064
AACGCAG49000.030.7873526
CAACGCA48750.030.5261125
GACAGGT2950.030.0370861
CGCAGAG51700.029.1136348
ACGCAGA51850.029.0294117
AGAGTAC52250.028.9302411
AATGGTA8300.028.8365270
CAGAGTA52550.028.76779710
TCTCGCG4800.028.70908570
GCAGAGT53100.028.4743069
CGGTCTT807.512432E-526.80997362
CTGTACT659.3389204E-426.1968274
GTATAAA1058.55247E-725.9661271
CAGGTAT2000.025.5604061
CCGTTAA801.0411047E-425.55396816
Produced by FastQC (version 0.11.5)