FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714921
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31610.44214675467639086No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACACCGTTCCTG25970.36325691929597814No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25730.3598999050244712No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACACCGTTCCTGCCTGCATATGAGCTG25720.3597600294298251No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25430.3557036371850876No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCACCATCTCCTG24980.3494092354260121No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23420.32758864266121707No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23150.32381200160577184No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC22280.31164282487155925No Hit
CATTATTACTGCAGCTCATATGCAGGCAGGAACGGTGTGTTATTCGGTGGAGGGACCAAACTGACCGTCCTAGGT21230.2969558874337164No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19010.2659035054222774No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18980.26548387863833905No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTACAACTCTGTCTCCTGGTACCAACAGCACCCAGGCAGAGC18290.25583246260775666No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17750.2482791804968661No Hit
TCTCTGGGCTCCTGGCTGAGGATGAGGCTCATTATTACTGCAGCTCATAT17550.24548166860394366No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17360.24282403230566735No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCAC17240.2411455251699139No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16760.23443149662690002No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16460.2302352287875164No Hit
GTAATAATGAGCCTCATCCTCAGCCAGGAGCCCAGAGACGGTCAGGGAGG16330.22841684605711682No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16080.22491995619096378No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15870.22198256870339517No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15640.2187654300265344No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15430.21582804253896581No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15330.21442928659250465No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15230.2130305306460434No Hit
GTGTTATTCGGTGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG14880.20813488483342915No Hit
GAATAACACACCGTTCCTGCCTGCATATGAGCTGCAGTAATAATGAGCCT14610.2043582437779839No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14490.20267973664223038No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14380.20114110510112307No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14080.1969448372617394No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13540.18939155515084882No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT12340.17260648379331422No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12210.17078810106291464No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11820.1653329528717159No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11740.16421394811454693No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACAC11580.16197593860020898No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGCGGGCCG11340.15861892432870206No Hit
TTATTACTGCAGCTCATATGCAGGCAGGAACGGTGTGTTATTCGGTGGAG11340.15861892432870206No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11310.1581992975447637No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCAC11140.15582141243577963No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11020.15414290530002614No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10860.15190489578568822No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT10730.15008651305528864No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10730.15008651305528864No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCACCATCT10690.14952701067670413No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTACAACTCTGTCTCCTGGTACCAACAGCA10680.14938713508205803No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10630.1486877571088274No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCACCATCTCCTGCA10420.14575036962125887No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTACAACTC10410.14561049402661272No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACACCGTTC10330.14449148926944375No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10320.14435161367479765No Hit
GGATGAGGCTCATTATTACTGCAGCTCATATGCAGGCAGGAACGGTGTGTTATTCGGTGGAGGGACCAAACTGAC10260.14351236010692092No Hit
GTTTGGTCCCTCCACCGAATAACACACCGTTCCTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCATCCTCAG10240.14323260891762865No Hit
CTCCTGGCCAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT10220.1429528577283364No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10170.1422534797551058No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10130.1416939773765213No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCA9820.13735783394249157No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACACCGTTCCTGC9750.1363787047799687No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9640.13484007323886135No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC9630.13470019764421523No Hit
GTGTGTTATTCGGTGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC9610.134420446454923No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT9570.13386094407633853No Hit
GTACCAACAGCACCCAGGCAGAGCCCCCAAGGTCATGATTTCTGACGTCA9560.13372106848169238No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9310.13022417861553934No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC9290.1299444274262471No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9260.12952480064230873No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9250.1293849250476626No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9230.12910517385837036No Hit
CTCCCTGACCGTCTCTGGGCTCCTGGCTGAGGATGAGGCTCATTATTACT9220.12896529826372424No Hit
GGTTACAACTCTGTCTCCTGGTACCAACAGCACCCAGGCAGAGCCCCCAAGGTCATGATTTCTGACGTCAGTCAG9210.12882542266907812No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9180.12840579588513976No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8940.12504878161363284No Hit
GTTCCTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCATCCTCAGCCAGGAGCCCAGAGACGGTCAGGGAGGC8780.12281077209929489No Hit
GGCAGGAACGGTGTGTTATTCGGTGGAGGGACCAAACTGACCGTCCTAGG8590.12015313580101858No Hit
GTTACAACTCTGTCTCCTGGTACCAACAGCACCCAGGCAGAGCCCCCAAG8550.11959363342243408No Hit
ATAACACACCGTTCCTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCA8520.11917400663849571No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8340.11665624593486554No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC8310.11623661915092717No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8220.11497773879911206No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT8190.1145581120151737No Hit
CCCTGACCGTCTCTGGGCTCCTGGCTGAGGATGAGGCTCATTATTACTGC8160.11413848523123533No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7880.11022196858114393No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7870.11008209298649782No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7770.10868333704003659No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG7760.10854346144539048No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCC7750.10840358585074435No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA7740.10826371025609823No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7700.10770420787751372No Hit
GAGTTGTAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGAC7570.10588582514711416No Hit
CCCAGGCAGAGCCCCCAAGGTCATGATTTCTGACGTCAGTCAGCGGCCCGCAGGGGTCCCTGATCGCTTCTCTGG7550.10560607395782191No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT7540.1054661983631758No Hit
GCAGTAATAATGAGCCTCATCCTCAGCCAGGAGCCCAGAGACGGTCAGGG7420.10378769122742232No Hit
CTCTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC7380.10322818884883785No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC7370.10308831325419171No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC7350.10280856206489948No Hit
GGTACCAACAGCACCCAGGCAGAGCCCCCAAGGTCATGATTTCTGACGTC7240.10126993052379214No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG7160.10015092576662317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2050.058.567970
CTCTCGT252.6111503E-455.00763337
GAGTGCG200.00776908851.0430689
TCTCGTA1900.047.09258738
CGTATGC2250.046.0773741
GTGGTAT2000.045.94841
TGGTATC1950.045.3811382
TCGTATG2300.045.01494240
ACGAGAC2500.043.57808322
ATGCCGT2500.043.0384344
GATCTCG1850.042.7031936
GGTATCA6400.042.5448151
ATCTCGT1900.041.6176237
CTTATAC2800.041.329251
CACGAGA2600.040.59256421
AGACCGA2550.040.08704825
GAGACCG2650.039.8378224
TTATACA2850.039.4099352
CCCACGA2600.039.2776319
TCTCCGA2700.039.0711
Produced by FastQC (version 0.11.5)