FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004686586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714921
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29360.4106747458810134No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26530.371089952596161No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26170.3660544311889006No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24500.34269520688299826No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACACCGTTCCTGCCTGCATATGAGCTG24450.34199582890976765No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCACCATCTCCTG23960.3351419247721077No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACACCGTTCCTG23880.3340229200149387No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC19290.2698200220723688No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCAC19190.2684212661259076No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18900.2643648738811701No Hit
CATTATTACTGCAGCTCATATGCAGGCAGGAACGGTGTGTTATTCGGTGGAGGGACCAAACTGACCGTCCTAGGT18700.26156736198824765No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18630.2605882328257248No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18400.257371094148864No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18290.25583246260775666No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTACAACTCTGTCTCCTGGTACCAACAGCACCCAGGCAGAGC18150.25387420428271096No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17780.24869880728080446No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16650.2328928650857927No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16380.2291162240303474No Hit
TCTCTGGGCTCCTGGCTGAGGATGAGGCTCATTATTACTGCAGCTCATAT16280.22771746808388618No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15800.22100343954087237No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15370.21498878897108908No Hit
GTGTTATTCGGTGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG14920.20869438721201364No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14710.20575699972444506No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14420.20170060747970756No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14170.19820371761355451No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13890.1942872009634631No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13740.19218906704377126No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13670.19120993788124843No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13600.19023080871872555No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13020.1821180242292505No Hit
GAATAACACACCGTTCCTGCCTGCATATGAGCTGCAGTAATAATGAGCCT12470.1744248665237138No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11710.16379432133060853No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11620.16253544097879347No Hit
GTAATAATGAGCCTCATCCTCAGCCAGGAGCCCAGAGACGGTCAGGGAGG11590.1621158141948551No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11330.15847904873405594No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT11150.15596128803042575No Hit
GTGTGTTATTCGGTGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC10920.15274414935356495No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACAC10920.15274414935356495No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10870.15204477138033434No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10630.1486877571088274No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10600.14826813032488903No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTACAACTCTGTCTCCTGGTACCAACAGCA10520.14714912556772006No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCACCATCT10340.1446313648640899No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10330.14449148926944375No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10280.14379211129621314No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10100.14127435059258295No Hit
CTCCTGGCCAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT9950.13917621667289112No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCAC9930.1388964654835989No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9930.1388964654835989No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9920.13875658988895276No Hit
TTATTACTGCAGCTCATATGCAGGCAGGAACGGTGTGTTATTCGGTGGAG9790.1369382071585532No Hit
GGATGAGGCTCATTATTACTGCAGCTCATATGCAGGCAGGAACGGTGTGTTATTCGGTGGAGGGACCAAACTGAC9780.13679833156390706No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT9660.1351198244281536No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9560.13372106848169238No Hit
GTTTGGTCCCTCCACCGAATAACACACCGTTCCTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCATCCTCAG9510.13302169050846177No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCA9360.13092355658876995No Hit
GTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTACAACTC9260.12952480064230873No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9220.12896529826372424No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9140.12784629350655527No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAACACACCGTTC9140.12784629350655527No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9110.1274266667226169No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9000.12588803518150957No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGCGGGCCG8960.12532853280292508No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC8960.12532853280292508No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT8960.12532853280292508No Hit
GGTTACAACTCTGTCTCCTGGTACCAACAGCACCCAGGCAGAGCCCCCAAGGTCATGATTTCTGACGTCAGTCAG8840.12365002566717162No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGCCAGTCAGTCACCATCTCCTGCA8690.12155189174747978No Hit
CTCCCTGACCGTCTCTGGGCTCCTGGCTGAGGATGAGGCTCATTATTACT8360.11693599712415777No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8180.11441823642052759No Hit
GTACCAACAGCACCCAGGCAGAGCCCCCAAGGTCATGATTTCTGACGTCA8030.11232010250083575No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8000.11190047571689739No Hit
CCCTGACCGTCTCTGGGCTCCTGGCTGAGGATGAGGCTCATTATTACTGC7980.11162072452760516No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC7940.11106122214902066No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7890.11036184417579005No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7640.10686495430963701No Hit
GTTCCTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCATCCTCAGCCAGGAGCCCAGAGACGGTCAGGGAGGC7610.10644532752569864No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCACCGAATAA7580.10602570074176028No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCC7550.10560607395782191No Hit
GGCAGGAACGGTGTGTTATTCGGTGGAGGGACCAAACTGACCGTCCTAGG7540.1054661983631758No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7480.10462694479529905No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7470.10448706920065294No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7460.10434719360600682No Hit
ATAACACACCGTTCCTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCA7390.10336806444348397No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7350.10280856206489948No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7320.10238893528096112No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7280.10182943290237663No Hit
GTTACAACTCTGTCTCCTGGTACCAACAGCACCCAGGCAGAGCCCCCAAG7250.10140980611843826No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA7170.10029080136126928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)